Analysis of 10 X-STRs in three African populations

Gomes, Iva; Alves, Cíntia; Maxzud, Karina; Pereira, Rui; Prata, Maria João; Sánchez-Diz, Paula; Carracedo, Angel; Amorim, António; Gusmão, Leonor

doi:10.1016/j.fsigen.2007.01.001

« Previous Next »Forensic Science International: Genetics
Volume 1, Issue 2 , Pages 208-211, June 2007

Analysis of 10 X-STRs in three African populations

Iva Gomes
- IPATIMUP, Institute of Pathology and Molecular Immunology of the University of Porto, Porto, Portugal
- Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain
- Corresponding author at: IPATIMUP, Institute of Pathology and Molecular Immunology of the University of Porto, Porto, Portugal. Tel.: +351 22 5570700; fax: +351 22 5570799.
Cíntia Alves
- IPATIMUP, Institute of Pathology and Molecular Immunology of the University of Porto, Porto, Portugal
Karina Maxzud
- CEPROCOR Unit, Cordoba Science Agency, Hospital Complex of Santa Maria of Punilla, Cordoba, Argentina
Rui Pereira
- IPATIMUP, Institute of Pathology and Molecular Immunology of the University of Porto, Porto, Portugal
Maria João Prata
- IPATIMUP, Institute of Pathology and Molecular Immunology of the University of Porto, Porto, Portugal
- Faculty of Sciences of the University of Porto, Porto, Portugal
Paula Sánchez-Diz
- Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain
Angel Carracedo
- Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain
António Amorim
- IPATIMUP, Institute of Pathology and Molecular Immunology of the University of Porto, Porto, Portugal
- Faculty of Sciences of the University of Porto, Porto, Portugal
Leonor Gusmão
- IPATIMUP, Institute of Pathology and Molecular Immunology of the University of Porto, Porto, Portugal

Received 25 January 2007; accepted 27 January 2007. published online 09 March 2007.

Abstract

Three African populations were genetically studied through a decaplex X chromosome short tandem repeat (STR) system, which includes the following loci: DXS8378, DXS9898, DXS8377, HPRTB, GATA172D05, DXS7423, DXS6809, DXS7132, DXS101 and DXS6789. A total of 237 unrelated male individuals from Angola, Mozambique and Uganda were typed. DXS8377 revealed to be the most polymorphic marker and in contrast, locus DXS7423 was the least informative in Angola and Mozambique and DXS8378 in Uganda. No significant associations between alleles of any pair of loci were found in these three population groups. The overall mean exclusion chances for the 10-plex in parentage testing, when both mother and daughter are investigated are above 1 in 4.2 million being the highest in Mozambique (1 in 5.3 million); in duos these values are approximately 1 in 60 thousand. Concerning the overall power of discrimination, this decaplex can discriminate 1 in nearly 41 million Ugandan men and 1 in around 30 million Angolan and Mozambican men; raising an order of magnitude of over 13 digits in all population groups for women. All these parameters demonstrate the potential of this decaplex for parentage testing as well as for identification purposes.

Keywords: Short tandem repeat, X chromosome, Africa