Forensic Science International: Genetics
Volume 1, Issue 2 , Pages 163-169 , June 2007

Real-time PCR detection of five different “endogenous control gene” transcripts in forensic autopsy material

Received 25 January 2007 ,Accepted 27 January 2007.

References 

  1. Bauer M, Polzin S, Patzelt D. Quantification of RNA degradation by semi-quantitative duplex and competitive RT-PCR: a possible indicator of the age of bloodstains?. Forens. Sci. Int. 2003;138:94–103
  2. Bauer M, Gramlich I, Polzin S, Patzelt D. Quantification of mRNA degradation as possible indicator of postmortem interval—a pilot study. Leg. Med. 2003;5:220–227
  3. Bauer M, Patzelt D. A method for simultaneous RNA and DNA isolation from dried blood and semen stains. Forens. Sci. Int. 2003;136:76–78
  4. Kobayashi H, Sakimura K, Kuwano R, Sato S, Ikuta F, Takahashi Y, et al. Stability of messenger RNA in postmortem human brains and construction of human brain cDNA libraries. J. Mol. Neurosci. 1990;2:29–34
  5. Preece P, Virley DJ, Costandi M, Coombes R, Moss SJ, Mudge AW, et al. An optimistic view for quantifying mRNA in post-mortem human brain. Brain Res. Mol. Brain Res. 2003;116:7–16
  6. Preece P, Cairns NJ. Quantifying mRNA in postmortem human brain: influence of gender, age at death, postmortem interval, brain pH, agonal state and inter-lobe mRNA variance. Brain Res. Mol. Brain Res. 2003;118:60–71
  7. Heinrich M, Matt K, Lutz-Bonengel S, Schmidt U. Successful RNA extraction from various human postmortem tissues. Int. J. Legal Med. 2007;121:136–142
  8. Bustin SA, Nolan T. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Tech. 2004;15:155–166
  9. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−delta deltaC(T)) method. Methods. 2001;25:402–408
  10. Huggett J, Dheda K, Bustin S, Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 2005;6:279–284
  11. Albrecht A, Gerling I, Henssge C, Hochmeister M, Kleiber M, Madea B, et al. Zur Anwendung des Rektaltemperatur-Todeszeit-Nomogramms am Leichenfundort. Z. Rechtsmed. 1990;103:257–278
  12. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002;3:RESEARCH0034
  13. Fleige S, Walf V, Huch S, Prgomet C, Sehm J, Pfaffl MW. Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR. Biotechnol. Lett. 2006;28:1601–1613
  14. Barrachina M, Castano E, Ferrer I. TaqMan PCR assay in the control of RNA normalization in human post-mortem brain tissue. Neurochem. Int. 2006;49:276–284
  15. Libus J, Storchova H. Quantification of cDNA generated by reverse transcription of total RNA provides a simple alternative tool for quantitative RT-PCR normalization. Biotechniques. 2006;41:156–160

PII: S1872-4973(07)00039-7

doi: 10.1016/j.fsigen.2007.01.002

Forensic Science International: Genetics
Volume 1, Issue 2 , Pages 163-169 , June 2007

Article Tools

* Image Usage & Resolution