Forensic application of the affymetrix human mitochondrial resequencing array☆
Received 22 January 2007; accepted 27 January 2007. published online 12 March 2007.
Abstract
In the field of forensic DNA testing, sequencing regions of the mitochondrial genome is performed when insufficient genomic DNA is present for traditional autosomal short tandem repeat (STR) testing. Sequencing coding region polymorphisms in the mitochondrial genome can be useful for resolving individuals who have the identical HV1 and HV2 control region sequence. Various methods and strategies have been established to interrogate coding region polymorphisms. These range from SNP assays probing sites most likely to differentiate individuals based on their HV1/HV2 sequence to the use of mass spectrometry to pyrosequencing. Here we evaluate the potential of the Affymetrix GeneChip Mitochondrial Resequencing Array (version 2.0) for forensic applications.
aNational Institute of Standards and Technology (NIST), Biochemical Sciences Division, 100 Bureau Dr., M/S 8311, Gaithersburg, MD 20899-8311, United States
bArmed Forces DNA Identification Laboratory, 1413 Research Boulevard, Building 101, Rockville, MD 20850, United States
☆ Contribution of the U.S. National Institute of Standards and Technology. Not subject to copyright. Points of view in this document are those of the authors and do not necessarily represent the official position or policies of the U.S. Department of Justice. Certain commercial equipment, instruments and materials are identified in order to specify experimental procedures as completely as possible. In no case does such identification imply a recommendation or endorsement by the National Institute of Standards and Technology nor does it imply that any of the materials, instruments or equipment identified are necessarily the best available for the purpose.
ABSTRACT