Forensic Science International: Genetics
Volume 1, Issue 2 , Pages 205-207, June 2007

Q8—A short amplicon multiplex including the German DNA database systems

Institute of Legal Medicine, University Hospital Ulm, Germany

Received 29 January 2007; accepted 3 February 2007. published online 24 March 2007.

Abstract 

We have developed a modified STR (short tandem repeat) concept including the eight German DNA database systems D3S1358, FGA, TH01, VWA, SE33, D8S1179, D18S51, D21S11 and the gender typing system Amelogenin (Q8). The Q8 is well suitable especially for weak and degraded stains. Compared to commercially established DNA database kits the amplicon lengths were significantly reduced. To optimise the typing success for weak stains the most sensitive fluorescent dyes FAM, JOE and TET were used.

By comparing the Q8 kit with the MPX2 kit, a well-established commercial kit, a higher success rate was reached using our concept.

Keywords: Short amplicon multiplex, DNA degradation, German DNA database systems

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PII: S1872-4973(07)00062-2

doi:10.1016/j.fsigen.2007.02.005

Forensic Science International: Genetics
Volume 1, Issue 2 , Pages 205-207, June 2007

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