New alleles and mutational events at 14 STR loci from different German populations

Becker, Dorit; Bender, Klaus; Edelmann, Jeanett; Götz, Frank; Henke, Lotte; Hering, Sandra; Hohoff, Carsten; Hoppe, Karolin; Klintschar, Michael; Muche, Matthias; Rolf, Burkhard; Szibor, Reinhard; Weirich, Volker; Jung, Martin; Brabetz, Werner

doi:10.1016/j.fsigen.2007.04.001

« Previous Next »Forensic Science International: Genetics
Volume 1, Issue 3 , Pages 232-237, December 2007

New alleles and mutational events at 14 STR loci from different German populations

Dorit Becker
- Biotype AG, Moritzburger Weg 67 D, 01109 Dresden, Germany
- Corresponding author. Tel.: +49 351 8838 128; fax: +49 351 8838 435.
Klaus Bender
- Institute of Legal Medicine, University of Mainz, Am Pulverturm 3, 55131 Mainz, Germany
Jeanett Edelmann
- Institute of Legal Medicine, University of Leipzig, Johannisallee 28, 04103 Leipzig, Germany
Frank Götz
- Qualitype AG, Moritzburger Weg 67, 01109 Dresden, Germany
Lotte Henke
- Institut für Blutgruppenforschung LGC GmbH, Hohenzollernring 57, 50672 Köln, Germany
Sandra Hering
- Institute of Legal Medicine, University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany
Carsten Hohoff
- Institute of Legal Medicine, University of Münster, Röntgenstrasse 23, 48149 Münster, Germany
Karolin Hoppe
- Institute of Legal Medicine, University of Münster, Röntgenstrasse 23, 48149 Münster, Germany
Michael Klintschar
- Institute of Legal Medicine, University of Halle, Franzosenweg 1, 06112 Halle, Germany
Matthias Muche
- Institute for Blood Group Serology and Genetics, Holsteinischer Kamp 67, 22081 Hamburg, Germany
Burkhard Rolf
- Institute of Legal Medicine, University of München, Frauenlobstrasse 7a, 80337 München, Germany
Reinhard Szibor
- Institute of Legal Medicine, University of Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany
Volker Weirich
- Institute of Legal Medicine, University of Rostock, St.-Georg-Strasse 108, 18055 Rostock, Germany
Martin Jung
- Biotype AG, Moritzburger Weg 67 D, 01109 Dresden, Germany
Werner Brabetz
- Biotype AG, Moritzburger Weg 67 D, 01109 Dresden, Germany

Received 30 January 2007; received in revised form 10 April 2007; accepted 11 April 2007. published online 02 June 2007.

Abstract

The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci.

Keywords: DNA typing, Short tandem repeat, Primer binding site mutation, Silent allele, Mutation rate