Forensic Science International: Genetics
Volume 1, Issue 3 , Pages 247-252 , December 2007

Identification and isolation of male cells using fluorescence in situ hybridisation and laser microdissection, for use in the investigation of sexual assault

Received 29 January 2007 ,Revised 7 May 2007 ,Accepted 15 May 2007.

References 

  1. Gill P, Jeffreys AJ, Werrett DJ. Forensic application of DNA ‘fingerprints’. Nature. 1985;318:577–579
  2. Pollak OJ. Semen and seminal stains. Arch. Pathol. 1943;35:140–196
  3. Willott GM. Frequency of azoospermia. Forensic Sci. Int. 1982;20(1):9–10
  4. Collins KA, Rao PN, Hayworth R, Schnell S, Tap MP, Lantz PE, et al. Identification of sperm and non-sperm male cells in cervicovaginal smears using fluorescence in situ hybridization: applications in alleged sexual assault cases. J. Forensic Sci. 1994;39(6):1347–1355
  5. Dziegelewski M, Simich JP, Rittenhouse-Olson K. Use of a Y chromosome probe as an aid in the forensic proof of sexual assault. J. Forensic Sci. 2002;47(3):601–604
  6. Rao PN, Collins KA, Geisinger KR, Parsons LH, Schnell S, Hayworth-Hodge R, et al. Identification of male epithelial cells in routine postcoital cervicovaginal smears using fluorescence in situ hybridization: application in sexual assault and molestation. Am. J. Clin. Pathol. 1995;104(1):32–35
  7. Prahlow JA, Lantz PE, Cox-Jones K, Rao PN, Pettenati MJ. Gender identification of human hair using fluorescence in situ hybridization. J. Forensic Sci. 1996;41(6):1035–1037
  8. Collins KA, Cina SJ, Pettenati MJ, Fitts M. Identification of female cells in postcoital penile swabs using fluorescence in situ hybridization. Arch. Pathol. Lab. Med. 2000;124(7):1080–1082
  9. Cina SJ, Collins KA, Fitts M, Pettenati MJ. Isolation and identification of male and female DNA on a postcoital condom. Arch. Pathol. Lab. Med. 2000;124(7):1083–1086
  10. Pettenati MJ, Rao PN, Schnell S, Hayworth-Hodge R, Lantz PE, Geisinger KR. Gender identification of dried human bloodstains using fluorescence in situ hybridization. J. Forensic Sci. 1995;40(5):885–887
  11. Di Martino D, Giuffre G, Staiti N, Simone A, Le Donne M, Savaro L. Single sperm cell isolation by laser microdissection. Forensic Sci. Int. 2004;146S:S151–S153
  12. Elliott K, Hill DS, Lambert C, Burroughes TR, Gill P. Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides. Forensic Sci. Int. 2003;137(1):28–36
  13. Sanders CT, Sanchez N, Ballantyne J, Peterson DA. Laser microdissection separation of pure spermatozoa from epithelial cells for short tandem repeat analysis. J. Forensic Sci. 2006;51(4):748–757
  14. Di Martino D, Giuffre G, Staiti N, Simone A, Todaro P, Savaro L. Laser microdissection and DNA typing of cells from single hair follicles. Forensic Sci. Int. 2004;146S:S155–S157
  15. Bauer M, Thalheimer A, Patzelt D. Paternity testing after pregnancy termination using laser microdissection of chorionic villi. Int. J. Legal Med. 2002;116(1):39–42
  16. Budimlija ZM, Lechpammer M, Popiolek D, Fogt F, Prinz M, Bieber FR. Forensic applications of laser capture microdissection: use in DNA-based parentage testing and platform validation. Croat. Med. J. 2005;46(4):549–555
  17. Robino C, Barilaro MR, Gino S, Chiarle R, Palestro G, Torre C. Incestuous paternity detected by STR-typing of chorionic villi isolated from archival formalin-fixed paraffin-embedded abortion material using laser microdissection. J. Forensic Sci. 2006;51(1):90–92
  18. Anslinger K, Mack B, Bayer B, Rolf B, Eisenmenger W. Digoxigenin labelling and laser capture microdissection of male cells. Int. J. Legal Med. 2005;119(6):374–377
  19. Anslinger K, Bayer B, Mack B, Eisenmenger W. Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling. Int. J. Legal Med. 2007;121:54–56
  20. Gill P, Whitaker J, Flaxman C, Brown N, Buckleton J. An investigation of the rigor of interpretation rules for STRs derived from less than 100pg of DNA. Forensic Sci. Int. 2000;112(1):17–40
  21. Cotton EA, Allsop RF, Guest JL, Frazier RRE, Koumi P, Callow IP, et al. Validation of the AMPFlSTR SGMPlus system for use in forensic casework. Forensic Sci. Int. 2000;112(2–3):151–161
  22. Davies A, Wilson E. The persistence of seminal constituents in the human vagina. Forensic Sci. 1974;3(1):45–55
  23. Shewale JG, Sikka SC, Schneida E, Sinha SK. DNA profiling of azoospermic semen samples from vasectomized males by using Y-Plex 6 amplification kit. J. Forensic Sci. 2003;48(1):127–129

PII: S1872-4973(07)00094-4

doi: 10.1016/j.fsigen.2007.05.003

Forensic Science International: Genetics
Volume 1, Issue 3 , Pages 247-252 , December 2007

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