Forensic Science International: Genetics
Volume 2, Issue 1 , Pages 54-60, January 2008

JC viral DNA chip allows geographical localization of unidentified cadavers for rapid identification

  • Hiroshi Ikegaya

      Affiliations

    • National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81 4 7135 8001; fax: +81 4 7133 9159.
    • ,
  • Hirotaro Iwase

      Affiliations

    • Department of Legal Medicine, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan
    • ,
  • Pekka J. Saukko

      Affiliations

    • Department of Forensic Medicine, University of Turku, Turku 20520, Finland
    • ,
  • Tomoko Akutsu

      Affiliations

    • National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan
    • ,
  • Koichi Sakurada

      Affiliations

    • National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan
    • ,
  • Mineo Yoshino

      Affiliations

    • National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan

Received 20 April 2007; received in revised form 19 July 2007; accepted 13 August 2007. published online 27 September 2007.

Abstract 

To narrow down the geographical origins of unidentified cadavers, a rapid and simple method to detect JC virus (JCV) genotypes using a DNA chip was developed.

Fifty-four probes on a 3mm silicon DNA chip were designed to distinguish 12 JCV genotypes. The detection limit of the DNA chip was 0.001pg/μL of template JCV DNA concentration, showing a sensitivity equal to that of the conventional method. The analysis requires only a few hours to yield results. This new DNA chip method helps to narrow the search area for human identification much more quickly and easily than use of the conventional method.

Keywords: JC virus, DNA chip, Unidentified cadaver, Geographic identification

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PII: S1872-4973(07)00117-2

doi:10.1016/j.fsigen.2007.08.009

Forensic Science International: Genetics
Volume 2, Issue 1 , Pages 54-60, January 2008

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