Forensic Science International: Genetics
Volume 2, Issue 3 , Pages 198-204 , June 2008

Resolving relationship tests that show ambiguous STR results using autosomal SNPs as supplementary markers

  • C. Phillips

      Affiliations

    • Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Spain
    • Genomic Medicine Group, Hospital Clinico Universitario, Santiago de Compostela, Spain
    • Corresponding Author InformationCorresponding author at: Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, 15704, Spain.
    • ,
  • M. Fondevila

      Affiliations

    • Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Spain
    • ,
  • M. García-Magariños

      Affiliations

    • Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Spain
    • ,
  • A. Rodriguez

      Affiliations

    • Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Spain
    • ,
  • A. Salas

      Affiliations

    • Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Spain
    • Genomic Medicine Group, Hospital Clinico Universitario, Santiago de Compostela, Spain
    • ,
  • Á. Carracedo

      Affiliations

    • Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Spain
    • Genomic Medicine Group, Hospital Clinico Universitario, Santiago de Compostela, Spain
    • ,
  • M.V. Lareu

      Affiliations

    • Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Spain
    • Genomic Medicine Group, Hospital Clinico Universitario, Santiago de Compostela, Spain

Received 9 January 2008 ,Revised 11 February 2008 ,Accepted 12 February 2008.

References 

  1. The STRbase website lists a wide range of observed STR mutation rates at: http://www.cstl.nist.gov/biotech/strbase/mutation.htm based on data from the most extensive recent AABB survey, typing trios with standard STRs, outlined at: http://www.aabb.org/Documents/Accreditation/Parentage_Testing_Accreditation_Program/ptannrpt03.pdf
  2. Brinkman B, Klintshar M, Neuhuber F, Hühne J, Rolf B. Mutation rates in human microsatellites: influence of the structure and length of the tandem repeat. Am. J. Hum. Genet. 1998;62:1408–1415
  3. A regularly updated catalogue of STR null alleles reported in the literature is maintained at STRbase: http://www.cstl.nist.gov/biotech/strbase/NullAlleles.htm
  4. Fimmers R, Henke L, Henke J, Baur MP. How to deal with mutations in DNA-testing. Adv. Forensic Haemogenet. 1992;4:285–287
  5. Amorim A, Alves C, Pereira L, Gusmão L. Genotyping inconsistencies and null alleles using AmpFlSTR® Identifiler® and Powerplex® 16 kits. Prog. Forensic Genet. 2004;10:176–178
  6. Mosibay A, Mozer TJ, Sprecher C. Promega corporation reveals primer sequences in its testing kits. J. Forensic Sci. 2000;45:1360–1362
  7. Lareu MV, Barral S, Salas A, Pestoni C, Carracedo Á. Sequence variation of a hypervariable short tandem repeat at the D1S1656 locus. Int. J. Legal Med. 1998;111:244–247
  8. Lareu MV, Pestoni C, Barros F, Salas A, Carracedo Á. Sequence variation of a hypervariable short tandem repeat at the D12S391 locus. Gene. 1996;182:151–153
  9. Lareu MV, Barral S, Salas A, Carracedo Á. Sequence variation of a variable short tandem repeat at the D18S535 locus. Int. J. Legal Med. 1998;111:337–339
  10. Sánchez JJ, Phillips C, Børsting C, Balogh K, Bogus M, Fondevila M, et al. A multiplex assay with 52 single nucleotide polymorphisms for human identification. Electrophoresis. 2006;27:1713–1724
  11. Fondevila M, Phillips C, Naverán N, Cerezo M, Rodríguez A, Calvo R, et al. Challenging DNA: assessment of a range of genotyping approaches for highly degraded forensic samples. Prog. Forensic Genet. 2008;12:(in publication)
  12. Fondevila M, Phillips C, Naveran N, Fernández L, Cerezo M, Salas A, et al. Identification of skeletal remains using short-amplicon marker analysis of severely degraded DNA extracted from a decomposed and charred femur. Forensic Sci. Int. Genet. 2008;2:212–218
  13. Phillips C, Fang R, Ballard D, Fondevila M, Harrison C, Hyland F, et al. The SNPforID Consortium, Evaluation of the Genplex SNP typing system and a 49plex forensic marker panel. Forensic Sci. Int. Genet. 2007;1:180–185
  14. Egeland T, Mostad P, Mevåg B, Stenersen M. Beyond traditional paternity and identification cases. Selecting the most probable pedigree. Forensic Sci. Int. 2000;110:47–59
  15. Børsting C, Sanchez JJ, Birk AH, Bruun HQ, Hallenberg C, Hansen AJ, et al. Comparison of paternity indices based on typing 15 STRs, 7 VNTRs and 52 SNPs in 50 Danish mother-child-father trios. Prog. Forensic Genet. 2006;11:436–438
  16. Nachman MW, Crowell SL. Estimate of the mutation rate per nucleotide in humans. Genetics. 2000;156:297–304

PII: S1872-4973(08)00035-5

doi: 10.1016/j.fsigen.2008.02.002

Forensic Science International: Genetics
Volume 2, Issue 3 , Pages 198-204 , June 2008

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