Forensic Science International: Genetics
Volume 3, Issue 1 , Pages 14-21, December 2008

Developmental validation of a real-time PCR assay for the simultaneous quantification of total human and male DNA

Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, United States

Received 17 March 2008; received in revised form 9 July 2008; accepted 10 July 2008. published online 11 September 2008.

Abstract 

Multiplex human short tandem repeat analysis demands reliable DNA quantification to consistently produce interpretable genotypes. The Plexor® HY System is a multiplex quantitative PCR assay to quantify total human and male DNA. We performed developmental validation of the Plexor® HY System to demonstrate the performance capabilities and limitations of the assay for forensic applications. Validation studies examined: (a) human specificity, (b) sensitivity, (c) quantification of degraded DNA, (d) impact of inhibitors, (e) male/female mixture and Y-assay male specificity, (f) reproducibility and concordance and (g) population studies.

Keywords: Forensic science, DNA quantification, Real-time PCR, QPCR

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PII: S1872-4973(08)00097-5

doi:10.1016/j.fsigen.2008.07.004

Forensic Science International: Genetics
Volume 3, Issue 1 , Pages 14-21, December 2008