Developmental validation of a real-time PCR assay for the simultaneous quantification of total human and male DNA

Multiplex human short tandem repeat analysis demands reliable DNA quantification to consistently produce interpretable genotypes. The Plexor® HY System is a multiplex quantitative PCR assay to quantify total human and male DNA. We performed developmental validation of the Plexor® HY System to demonstrate the performance capabilities and limitations of the assay for forensic applications. Validation studies examined: (a) human specificity, (b) sensitivity, (c) quantification of degraded DNA, (d) impact of inhibitors, (e) male/female mixture and Y-assay male specificity, (f) reproducibility and concordance and (g) population studies.