Forensic Science International: Genetics
Volume 3, Issue 1 , Pages 42-45 , December 2008

Demonstration of rapid multiplex PCR amplification involving 16 genetic loci

Received 4 August 2008 ,Revised 10 September 2008 ,Accepted 14 September 2008.

References 

  1. Butler JM. Forensic DNA Typing. 2nd edition. New York: Elsevier; 2005;
  2. Butler JM. Genetics and genomics of core STR loci used in human identity testing. J. Forensic Sci. 2006;51:253–265
  3. Krenke BE, Tereba A, Anderson SJ, Buel E, Culhane S, Finis CJ, et al. Validation of a 16-locus fluorescent multiplex system. J. Forensic Sci. 2002;47:773–785
  4. Collins PJ, Hennessy LK, Leibelt CS, Roby RK, Reeder DJ, Foxall PA. Developmental validation of a single-tube amplification of the 13 CODIS STR loci, D2S1338, D19S433, and amelogenin: the AmpFlSTR Identifiler PCR Amplification Kit. J. Forensic Sci. 2004;49:1265–1277
  5. Belgrader P, Benett W, Hadley D, Richards J, Stratton P, Mariella R, et al. PCR detection of bacteria in seven minutes. Science. 1999;284:449–450
  6. Easley CJ, Karlinsey JM, Bienvenue JM, Legendre LA, Roper MG, Feldman SH, et al. A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability. Proc. Natl. Acad. Sci. U.S.A. 2006;103:19272–19277
  7. Liu P, Seo TS, Beyor N, Shin KJ, Scherer JR, Mathies RA. Integrated portable polymerase chain reaction-capillary electrophoresis microsystem for rapid forensic short tandem repeat typing. Anal. Chem. 2007;79:1881–1889
  8. Roper MG, Easley CJ, Landers JP. Advances in polymerase chain reaction on microfluidic chips. Anal. Chem. 2005;77:3887–3893
  9. Horsman KM, Bienvenue JM, Blasier KR, Landers JP. Forensic DNA analysis on microfluidic devices: a review. J. Forensic Sci. 2007;52:784–799
  10. Butler JM, Schoske R, Vallone PM, Redman JW, Kline MC. Allele frequencies for 15 autosomal STR loci on U.S. Caucasian, African American, and Hispanic populations. J. Forensic Sci. 2003;48:908–911
  11. Hill CR, Kline MC, Mulero JJ, Lagace RE, Chang C-W, Hennessy LK, et al. Concordance study between the AmpFlSTR MiniFiler PCR Amplification Kit and conventional STR typing kits. J. Forensic Sci. 2007;52:870–873
  12. Belgrader P, Smith JK, Weedn VW, Northrup MA. Rapid PCR for identity testing using a battery-powered miniature thermal cycler. J. Forensic Sci. 1998;43:315–319
  13. Brownstein MJ, Carpten JD, Smith JR. Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. BioTechniques. 1996;20:1004–1010
  14. Hill CR, Coble MD, Butler JM. Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples. J. Forensic Sci. 2008;53:73–80

 Certain commercial equipment, instruments and materials are identified in order to specify experimental procedures as completely as possible. In no case does such identification imply a recommendation or endorsement by the National Institute of Standards and Technology nor does it imply that any of the materials, instruments or equipment identified are necessarily the best available for the purpose.

PII: S1872-4973(08)00134-8

doi: 10.1016/j.fsigen.2008.09.005

Forensic Science International: Genetics
Volume 3, Issue 1 , Pages 42-45 , December 2008