Forensic Science International: Genetics
Volume 3, Issue 2 , Pages 89-95, March 2009

COI sequence variability between Chrysomyinae of forensic interest

National Institute for Criminalistics and Criminology, Vilvoordsesteenweg 100, 1120 Brussels, Belgium

Received 25 August 2008; received in revised form 27 October 2008; accepted 14 November 2008. published online 16 January 2009.

Abstract 

About 50 Chrysomyinae specimens belonging to three forensic relevant species (Chrysomia albiceps, Phormia regina and Protophormia terraenovae) were collected from different geographical locations in Belgium over the last 5yr. A 304-bp fragment of their mitochondrial COI gene is sequenced. The monophyletic branches of the phylogenetic tree reveal that this marker is suitable for discrimination between these species. The intra versus interspecific variability marks clear threshold levels for DNA barcoding. Nineteen Chrysomyinae specimens, collected from four locations in France, show mitotypes that are identical or at least very similar to the Belgian mitotypes. Considering additional specimens from outside of Europe reveals no intraspecific geographical variation within C. albiceps and P. terraenovae, whereas P. regina is subbranched in a Belgian–French and a USA–Chinese population.

Keywords: Forensic entomology, Mitochondrial DNA, Cytochrome oxidase subunit I, Chrysomyinae, Species determination

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PII: S1872-4973(08)00177-4

doi:10.1016/j.fsigen.2008.11.002

Forensic Science International: Genetics
Volume 3, Issue 2 , Pages 89-95, March 2009

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