Simplified buccal DNA extraction with FTA® Elute Cards

de Vargas Wolfgramm, Eldamária; de Carvalho, Fernanda Magri; da Costa Aguiar, Vitor Rezende; De Nadai Sartori, Mariana Penha; Hirschfeld-Campolongo, Gabriela C.R.; Tsutsumida, Weslley M.; Louro, Iúri Drumond

doi:10.1016/j.fsigen.2008.11.008

« Previous Next »Forensic Science International: Genetics
Volume 3, Issue 2 , Pages 125-127, March 2009

Simplified buccal DNA extraction with FTA^® Elute Cards

Eldamária de Vargas Wolfgramm
- Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
Fernanda Magri de Carvalho
- Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
Vitor Rezende da Costa Aguiar
- Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
Mariana Penha De Nadai Sartori
- Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
Gabriela C.R. Hirschfeld-Campolongo
- Applied Biosystems do Brasil, Av. do Café 277, 1° Andar, Bloco A, São Paulo, SP, Brazil
- Tel.: +55 800 7049004; fax: +55 5070 9662.
Weslley M. Tsutsumida
- Applied Biosystems do Brasil, Av. do Café 277, 1° Andar, Bloco A, São Paulo, SP, Brazil
- Tel.: +55 800 7049004; fax: +55 5070 9662.
Iúri Drumond Louro
- Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
- Corresponding author. Tel.: +55 27 3335 7251; fax: +55 27 3335 7250.

Received 12 August 2008; received in revised form 27 October 2008; accepted 24 November 2008. published online 16 January 2009.

Abstract

DNA isolation is the initial step of most genetic studies, and ideally it should use a reliable and non-invasive method. Buccal samples are adequate for such purposes, being painless, easy to collect and a very reliable DNA source. FTA^® Elute Cards are relatively new on the market and are designed for rapid blood DNA extraction, in which DNA is solubilized in water, instead of attached to the paper matrix. In this study, we sought to test if FTA^® Elute Cards are suitable for buccal DNA extraction. Furthermore, several time and temperature conditions were analyzed in order to determine the best concentration/time ratio. Twenty-five different conditions were tested in quadruplicate and extracted DNA was quantified by real-time PCR.

Keywords: Buccal DNA extraction, FTA^® Elute Cards, DNA concentration