Forensic Science International: Genetics
Volume 3, Issue 2 , Pages 125-127 , March 2009

Simplified buccal DNA extraction with FTA® Elute Cards

  • Eldamária de Vargas Wolfgramm

      Affiliations

    • Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
    • ,
  • Fernanda Magri de Carvalho

      Affiliations

    • Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
    • ,
  • Vitor Rezende da Costa Aguiar

      Affiliations

    • Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
    • ,
  • Mariana Penha De Nadai Sartori

      Affiliations

    • Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
    • ,
  • Gabriela C.R. Hirschfeld-Campolongo

      Affiliations

    • Applied Biosystems do Brasil, Av. do Café 277, 1° Andar, Bloco A, São Paulo, SP, Brazil
    • Tel.: +55 800 7049004; fax: +55 5070 9662.
    • ,
  • Weslley M. Tsutsumida

      Affiliations

    • Applied Biosystems do Brasil, Av. do Café 277, 1° Andar, Bloco A, São Paulo, SP, Brazil
    • Tel.: +55 800 7049004; fax: +55 5070 9662.
    • ,
  • Iúri Drumond Louro

      Affiliations

    • Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
    • Corresponding Author InformationCorresponding author. Tel.: +55 27 3335 7251; fax: +55 27 3335 7250.

Received 12 August 2008 ,Revised 27 October 2008 ,Accepted 24 November 2008.

References 

  1. Hansen TvO, Simonsen MK, Nielsen FC, Hundrup YA. Collection of blood, saliva, and buccal cell samples in a pilot study on the Danish nurse cohort: comparison of the response rate and quality of genomic DNA. Cancer Epidemiol. Biomarkers Prev. 2007;16:2072–2076
  2. Ng DPK, Koh D, Choo S, Chia K-S. Saliva as a viable alternative source of human genomic DNA in genetic epidemiology. Clin. Chim. Acta. 2006;367:81–85
  3. Milne E, van Bockxmeer FM, Robertson L, Brisbane JM, Ashton LJ, Scott RJ, et al. Buccal DNA collection: comparison of buccal swabs with FTA cards. Cancer Epidemiol. Biomarkers Prev. 2006;15:816–819
  4. Harty LC, Garcia-Closas M, Rothman N, Reid YA, Tucker MA, Hartge P. Collection of buccal cell DNA using treated cards. Cancer Epidemiol. Biomarkers Prev. 2000;9:501–506
  5. Fujita Y, Kubo S-I. Application of FTA technology to extraction of sperm DNA from mixed body fluids containing semen. Legal Med. 2006;8:43–47
  6. Tack LC, Thomas M, Reich K. Automated forensic DNA purification optimized for FTA card punches and identifiler STR-based PCR analysis. Clin. Lab. Med. 2007;27:183–191
  7. Lema C, Kohl-White K, Lewis LR, Dao DD. Optimized pH method for DNA elution from buccal cells collected in Whatman FTA cards. Genet. Test. 2006;10:126–130
  8. He H, Argiro L, Dessein H, Chevillard C. Improved technique that allows the performance of large-scale SNP genotyping on DNA immobilized by FTA® technology. Infect. Genet. Evol. 2007;7:128–132
  9. Pezzoli N, Silvy M, Woronko A, Le Treut T, Lévy-Mozziconacci A, Reviron D, et al. Quantification of mixed chimerism by real time PCR on whole blood-impregnated FTA cards. Leuk. Res. 2007;31:1175–1183
  10. Beckett SM, Laughton SJ, Pozza LD, McCowage GB, Marshall G, Cohn RJ, et al. Buccal swabs and treated cards: methodological considerations for molecular epidemiologic studies examining pediatric populations. Am. J. Epidemiol. SW. 2008;1–8
  11. Smith LM, Burgoyne LA. Collecting, archiving and processing DNA from wildlife samples using FTA® databasing paper. BMC Ecol. 2004;4:1–11
  12. Cao W, Hashibe M, Rao J-Y, Morgenstern H, Zhang Z-F. Comparison of methods for DNA extraction from paraffin-embedded tissues and buccal cells. Cancer Detect. Prev. 2003;27:397–404
  13. Walker AH, Najarian D, White DL, Jaffe JM, Kanetsky PA, Rebbeck TR. Collection of genomic DNA by buccal swabs for polymerase chain reaction-based biomarker assays. Environ. Health Perspect. 1999;107:517–520
  14. Quinque D, Kittler R, Kayser M, Stoneking M, Nasidze I. Evaluation of saliva as a source of human DNA for population and association studies. Anal. Biochem. 2006;353:272–277

PII: S1872-4973(08)00185-3

doi: 10.1016/j.fsigen.2008.11.008

Forensic Science International: Genetics
Volume 3, Issue 2 , Pages 125-127 , March 2009

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