Forensic Science International: Genetics
Volume 4, Issue 2 , Pages 68-72 , February 2010

Genetic profiles and sex identification of found-dead wolves determined by the use of an 11-loci PCR multiplex

  • Barbara van Asch

      Affiliations

    • Instituto de Patologia e Imunologia da Universidade do Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal
    • Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal
    • Corresponding Author InformationCorresponding author at: Instituto de Patologia e Imunologia da Universidade do Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal. Tel.: +351 225570700; fax: +351 225570799.
    • ,
  • Cíntia Alves

      Affiliations

    • Instituto de Patologia e Imunologia da Universidade do Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal
    • ,
  • Liliana Santos

      Affiliations

    • Instituto de Patologia e Imunologia da Universidade do Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal
    • Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal
    • ,
  • Raquel Pinheiro

      Affiliations

    • Instituto de Patologia e Imunologia da Universidade do Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal
    • Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal
    • ,
  • Filipe Pereira

      Affiliations

    • Instituto de Patologia e Imunologia da Universidade do Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal
    • Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal
    • ,
  • Leonor Gusmão

      Affiliations

    • Instituto de Patologia e Imunologia da Universidade do Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal
    • ,
  • António Amorim

      Affiliations

    • Instituto de Patologia e Imunologia da Universidade do Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal
    • Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal

Received 16 March 2009 ,Revised 30 April 2009 ,Accepted 22 May 2009.

References 

  1. Tsuda K, Kikkawa Y, Yonekawa H, Tanabe Y. Extensive interbreeding occurred among multiple matriarchal ancestors during the domestication of dogs: evidence from inter- and intraspecies polymorphisms in the D-loop region of mitochondrial DNA between dogs and wolves. Gen. Genet. Syst. 1997;72:229–238
  2. Vonholdt BM, Stahler DR, Smith DW, Earl DE, Pollinger JP, Wayne RK. The genealogy and genetic viability of reintroduced Yellowstone grey wolves. Mol. Ecol. 2008;17:252–274
  3. Lucchini V, Galov A, Randi E. Evidence of genetic distinction and long-term population decline in wolves (Canis lupus) in the Italian Apennines. Mol. Ecol. 2004;13:523–536
  4. Flagstad Ø, Walker CW, Vilà C, Sundqvist A-K, Fernholm B, Hufthammer AK, et al. Two centuries of the Scandinavian wolf population: patterns of genetic variability and migration during an era of dramatic decline. Mol. Ecol. 2003;12:869–880
  5. Verardi A, Lucchini V, Randi E. Detecting introgressive hybridization between free-ranging domestic dogs and wild wolves (Canis lupus) by admixture linkage disequilibrium analysis. Mol. Ecol. 2006;15:2845–2855
  6. Vilà C, Walker C, Sundqvist A-K, Flagstad Ø, Andersone Z, Casulli A, et al. Combined use of maternal, paternal e bi-parental genetic markers for the identification of dog wolf hybrids. Heredity. 2003;90:17–24
  7. Sundqvist A-K, Ellegren H, Vilà C. Wolf or dog? Genetic identification of predators from saliva collected around bite wounds on prey. Conserv. Genet. 2008;9:1275–1279
  8. Savolainen P, Lundeberg J. Forensic evidence based on mtDNA from dog and wolf hairs. J. For. Sci. 1999;44:77–81
  9. van Asch B, Alves C, Gusmão L, Pereira V, Pereira F, Amorim A. A new autosomal STR nineplex for canine identification and parentage testing. Electrophoresis. 2009;30:417–423
  10. UCSC Genome Browser, http://genome.ucsc.edu/.
  11. Eichmann C, Berger B, Steinlechner M, Parson W. Estimating the probability of identity in a random dog population using 15 highly polymorphic canine STR markers. For. Sci. Int. 2005;151:37–44
  12. Vallone PM, Butler JM. AutoDimer: a screening tool for primer–dimer and hairpin structures. Biotechnology. 2004;37:226–231
  13. Excoffier L, Laval G, Schneider S. Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol. Bioinf. Online. 2005;1:47–50
  14. I.W. Evett, B.S. Weir, Interpreting DNA Evidence: Statistical Genetics for Forensic Scientists, Sinauer Associates, Sunderland MA, USA, 1998.
  15. Pritchard JK, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data. Genetics. 2000;155:945–959
  16. V. Pimenta, I. Barroso, Situação populacional do lobo em Portugal: resultados do censo nacional 2002/2003, Relatório Técnico, Instituto de Conservação da Natureza/Grupo Lobo, Lisboa, Portugal, 2005, pp. 158.
  17. B.S. Weir, Multiple tests, in: Genetic Data Analysis II, Sinauer Associates, Sunderland MA, USA, 1996, pp. 134.

PII: S1872-4973(09)00083-0

doi: 10.1016/j.fsigen.2009.05.003

Forensic Science International: Genetics
Volume 4, Issue 2 , Pages 68-72 , February 2010

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