Forensic Science International: Genetics
Volume 4, Issue 2 , Pages 89-94 , February 2010

UV irradiation and autoclave treatment for elimination of contaminating DNA from laboratory consumables

Received 27 February 2009 ,Revised 14 May 2009 ,Accepted 16 June 2009.

References 

  1. Sullivan K, Johnson P, Rowlands D, Allen H. New developments and challenges in the use of the UK DNA Database: addressing the issue of contaminated consumables. Forensic Sci Int. 2004;146S:S175–S176
  2. D. Figarelli, DNA amplification, DNA Analyst Training http://www.nfstc.org/pdi/Subject04/pdi_s04_m04_01_b.htm.
  3. Dwyer DE, Saksena N. Failure of ultra-violet irradiation and autoclaving to eliminate PCR contamination. Mol Cell Probes. 1992;6:87–88
  4. Tamariz J, Voynarovska K, Prinz M, Caragine T. The application of ultraviolet irradiation to exogenous sources of DNA in plasticware and water for the amplification of low copy number DNA. J Forensic Sci. 2006;51:790–794
  5. Hall A, Ballantyne J. Characterization of UVC-induced DNA damage in bloodstains: forensic implications. Anal Bioanal Chem. 2004;380:72–83
  6. Rutty GN, Hopwood A, Tucker V. The effectiveness of protective clothing in the reduction of potential DNA contamination of the scene of crime. Int J Legal Med. 2003;117:170–174
  7. Swah K, Sesardić I, Bristol N, Ames C, Dagnall K, Ellis C, et al. Comparison of the effects of sterilization techniques on subsequent DNA profiling. Int J Legal Med. 2008;122:29–33
  8. http://www.merriam-webster.com/
  9. Linquist V, Stoddart CA, McCune JM. UV irradiation of polystyrene pipets releases PCR inhibitors. Biotechniques. 1998;24:50–52
  10. Burgess LC, Hall JO. UV light irradiation of plastic reaction tubes inhibits PCR. Biotechniques. 1999;27:252–256(tables and figures)

PII: S1872-4973(09)00098-2

doi: 10.1016/j.fsigen.2009.06.008

Forensic Science International: Genetics
Volume 4, Issue 2 , Pages 89-94 , February 2010

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