Forensic Science International: Genetics
Volume 4, Issue 2 , Pages 115-121 , February 2010

Post-coital vaginal sampling with nylon flocked swabs improves DNA typing

  • Corina C.G. Benschop

      Affiliations

    • Department of Biology (R&D), Netherlands Forensic Institute, P.O. Box 24044, 2490 AA The Hague, The Netherlands
  • ,
  • Danielle C. Wiebosch

      Affiliations

    • Department of Biology (R&D), Netherlands Forensic Institute, P.O. Box 24044, 2490 AA The Hague, The Netherlands
  • ,
  • Ate D. Kloosterman

      Affiliations

    • Department of Biology (R&D), Netherlands Forensic Institute, P.O. Box 24044, 2490 AA The Hague, The Netherlands
    • Institute for Biodiversity and Ecosystem Dynamics, Master in Forensic Science Program, Amsterdam Professional School of Science, University of Amsterdam, Amsterdam, The Netherlands
  • ,
  • Titia Sijen

      Affiliations

    • Department of Biology (R&D), Netherlands Forensic Institute, P.O. Box 24044, 2490 AA The Hague, The Netherlands
    • Corresponding Author InformationCorresponding author. Tel.: +31 708886888; fax: +31 708886555.

Received 15 April 2009 ,Revised 30 June 2009 ,Accepted 17 July 2009.

References 

  1. Voorhees JC, Ferrance JP, Landers JP. Enhanced elution of sperm from cotton swabs via enzymatic digestion for rape kit analysis. J. Forensic Sci. 2006;51:574–579
  2. Norris JV, Manning K, Linke SJ, Ferrance JP, Landers JP. Expedited, chemically enhanced sperm cell recovery from cotton swabs for rape kit analysis. J. Forensic Sci. 2007;52:800–805
  3. Allard JE, Baird A, Davidson G, Jones S, Lewis J, McKenna L, et al. A comparison of methods used in the UK and Ireland for the extraction and detection of semen on swabs and cloth samples. Sci. Justice. 2007;47:160–167
  4. Elliott K, Hill DS, Lambert C, Burroughes TR, Gill P. Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides. Forensic Sci. Int. 2003;137:28–36
  5. Sanders CT, Sanchez N, Ballantyne J, Peterson DA. Laser microdissection separation of pure spermatozoa from epithelial cells for short tandem repeat analysis. J. Forensic Sci. 2006;51:748–757
  6. Vandewoestyne M, Van Hoofstat D, Van Nieuweburgh F, Deforce D. Automatic detection of spermatozoa for laser capture microdissection. Int. J. Legal Med. 2007;123:169–175
  7. Collins KA, Rao PN, Hayworth R, Schnell S, Tap MP, Lantz PE, et al. Identification of sperm and non-sperm male cells in cervicovaginal smears using fluorescence in situ hybridization: applications in alleged sexual assault cases. J. Forensic Sci. 1994;39:1347–1355
  8. Cina MS, Collins KA, Fitts M, Pettenati MJ. Isolation and identification of male and female DNA on a postcoital condom. Arch. Pathol. Lab. Med. 2000;124:1083–1086
  9. Rao PN, Collins KA, Geisinger KR, Parsons LH, Schnell S, Hayworth-Hodge R, et al. Identification of male epithelial cells in routine postcoital cervicovaginal smears using fluorescence in situ hybridization. Am. J. Clin. Pathol. 1995;104:32–35
  10. Dziegelewski M, Simich JP, Rittenhouse-Olson K. Use of a Y chromosome probe as an aid in the forensic proof of sexual assault. J. Forensic Sci. 2002;47:601–604
  11. Anslinger K, Bayer B, Mack B, Eisenmenger W. Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling. Int. J. Legal Med. 2007;121:54–56
  12. Murray C, McAllister C, Elliott K. Identification and isolation of male cells using in situ hybridisation and laser microdissection, for use in the investigation of sexual assault. Forensic Sci. Int. 2007;1:247–252
  13. http://www.copanswabs.com/products/forensics/ (2008).
  14. Westen AA, Nagel JH, Benschop CC, Weiler NE, de Jong BJ, Sijen T. Higher capillary electrophoresis injection settings as an efficient approach to increase the sensitivity of STR typing. J. Forensic Sci. 2009;54:591–598
  15. Mauck CK, Doncel GF. Biomarkers of semen in the vagina: applications in clinical trials of contraception and prevention of sexually transmitted pathogens including HIV. Contraception. 2007;75:407–419
  16. Rupp JC. Sperm survival and prostatic acid phosphatase activity in victims of sexual assault. J. Forensic Sci. 1969;14:177–183
  17. Davies A, Wilson E. The persistence of seminal constituents in the human vagina. Forensic Sci. 1974;3:45–55
  18. Hall A, Ballantyne J. Novel Y-STR typing strategies reveal the genetic profile of the semen donor in extended interval post-coital cervicovaginal samples. Forensic Sci. Int. 2003;136:58–72
  19. Rogers D, Newton M. Evidence-based forensic sampling—more questions than answers. J. Clin. Forensic Med. 2006;13:162–163
  20. Mayntz-Press KA, Sims LM, Hall A, Ballantyne J. Y-STR profiling in extended interval (> or =3 days) postcoital cervicovaginal samples. J. Forensic Sci. 2008;53:342–348
  21. Morrison AI. Persistence of spermatozoa in the vagina and cervix. Br. J. Vener. Dis. 1972;48:141–143
  22. Silverman EM, Silverman AG. Persistence of spermatozoa in the lower genital tracts of women. JAMA. 1978;240:1875–1877
  23. Eungprabhanth V. Finding of the spermatozoa in the vagina related to elapsed time of coitus. Z. Rechtsmed. 1974;74:301–304
  24. Willot GM, Allard JE. Spermatozoa—their persistence after sexual intercourse. Forensic Sci. Int. 1982;19:135–154
  25. Apostolov A, Hristov S, Angelova E. DNA identification of biological traces and interpretation in a sexual assault case. Am. J. Forensic Med. Pathol. 2009;30:57–60
  26. http://www.swissforensix.com (2009).
  27. Shaw K, Sesardic I, Bristol N, Ames C, Dagnall K, Ellis C, et al. Comparison of the effects of sterilisation techniques on subsequent DNA profiling. Int. J. Legal Med. 2008;122:29–33

PII: S1872-4973(09)00121-5

doi: 10.1016/j.fsigen.2009.07.003

Forensic Science International: Genetics
Volume 4, Issue 2 , Pages 115-121 , February 2010