Forensic Science International: Genetics
Volume 4, Issue 3 , Pages 168-177, April 2010

Hydrolysis of DNA and its molecular components in the dry state

  • April Marrone

      Affiliations

    • Graduate Program in Chemistry, University of Central Florida, Orlando, FL, USA
    • Department of Chemistry, University of Central Florida, Orlando, FL, USA
    • National Center for Forensic Science, Orlando, FL, USA
    • ,
  • Jack Ballantyne

      Affiliations

    • Graduate Program in Chemistry, University of Central Florida, Orlando, FL, USA
    • Department of Chemistry, University of Central Florida, Orlando, FL, USA
    • National Center for Forensic Science, Orlando, FL, USA
    • Corresponding Author InformationCorresponding author at: National Center for Forensic Science, P.O. Box 162367, Orlando, FL 32816-2367, United States. Tel.: +1 407 823 4440; fax: +1 407 823 2252.

Received 7 April 2009; received in revised form 9 August 2009; accepted 11 August 2009. published online 11 September 2009.

Abstract 

The biochemistry of dry state DNA is of interest to the field of forensic biology. The precise chemical nature of the hydrolytic degradation products of the DNA molecule in the dry state has not been previously investigated in detail. In this study we found that the mechanistic chemistry of DNA hydrolysis appears to be the same for the hydrated and dry states. The thermodynamic parameters are also similar in both states and the activation energies for base hydrolysis are indistinguishable. The principal difference between the two states is the rate at which hydrolytic degradation occurs. The duplex configuration of dry state DNA offers much more protection for the molecule than is offered in single strand and nucleotide species. Single strand breaks of dry state duplex DNA occur with a half life of 24±2 days at 65°C and appear to occur in a mechanistic manner.

Keywords: Hydrolysis of DNA, Dry state DNA, Depurination

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PII: S1872-4973(09)00136-7

doi:10.1016/j.fsigen.2009.08.007

Forensic Science International: Genetics
Volume 4, Issue 3 , Pages 168-177, April 2010

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