Evaluation of seven X-chromosomal short tandem repeat loci located within the Xq26 region

Received 20 January 2009; received in revised form 17 July 2009; accepted 20 August 2009. published online 22 September 2009.

Abstract

In this study a set of 29 X-chromosomal short tandem repeats (STRs) located within the Xq26 region was evaluated. These STRs were found within the 133.14–133.45Mb region around the HPRTB locus. Evaluation of the microsatellites was performed with regard to polymorphism, reliable amplification, and low stutter artefacts. DXS10101, DXS10102, and DXS10103 were identified as those X-STRs with highest diversity; i.e. PIC values of 0.7174–0.8933. The locus DXS10101 was the optimal candidate for the integration in the commercial available test system Mentype Argus X-8 PCR amplification kit.

Keywords: STR, X-chromosome, Xq26, DXS10101, Mentype Argus X-8 PCR amplification kit

a Biotype AG, Moritzburger Weg 67, 01109 Dresden, Germany

b Qualitype AG, Moritzburger Weg 67, 01109 Dresden, Germany

c Institut für Rechtsmedizin, Universitätsklinikum Hamburg-Eppendorf, Butenfeld 34, 22529 Hamburg, Germany

d Institut für Rechtsmedizin, Universität Leipzig, Johannisallee 28, 04103 Leipzig, Germany

e Institut für Rechtsmedizin, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, Germany

f Institut für Rechtsmedizin, Otto-von-Guericke-Universität Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany

Corresponding author at: Biotype AG, Research and Development, Moritzburger Weg 67, 01109 Dresden, Germany. Tel.: +49 351 8838400; fax: +49 351 8838403.

PII: S1872-4973(09)00140-9

doi:10.1016/j.fsigen.2009.08.010

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