Evaluation of seven X-chromosomal short tandem repeat loci located within the Xq26 region

Rodig, Heike; Kloep, Frank; Weißbach, Lydia; Augustin, Christa; Edelmann, Jeannett; Hering, Sandra; Szibor, Reinhard; Götz, Frank; Brabetz, Werner

doi:10.1016/j.fsigen.2009.08.010

« Previous Next »Forensic Science International: Genetics
Volume 4, Issue 3 , Pages 194-199, April 2010

Evaluation of seven X-chromosomal short tandem repeat loci located within the Xq26 region

Heike Rodig
- Biotype AG, Moritzburger Weg 67, 01109 Dresden, Germany
Frank Kloep
- Biotype AG, Moritzburger Weg 67, 01109 Dresden, Germany
Lydia Weißbach
- Qualitype AG, Moritzburger Weg 67, 01109 Dresden, Germany
Christa Augustin
- Institut für Rechtsmedizin, Universitätsklinikum Hamburg-Eppendorf, Butenfeld 34, 22529 Hamburg, Germany
Jeannett Edelmann
- Institut für Rechtsmedizin, Universität Leipzig, Johannisallee 28, 04103 Leipzig, Germany
Sandra Hering
- Institut für Rechtsmedizin, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, Germany
Reinhard Szibor
- Institut für Rechtsmedizin, Otto-von-Guericke-Universität Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany
Frank Götz
- Qualitype AG, Moritzburger Weg 67, 01109 Dresden, Germany
Werner Brabetz
- Biotype AG, Moritzburger Weg 67, 01109 Dresden, Germany
- Corresponding author at: Biotype AG, Research and Development, Moritzburger Weg 67, 01109 Dresden, Germany. Tel.: +49 351 8838400; fax: +49 351 8838403.

Received 20 January 2009; received in revised form 17 July 2009; accepted 20 August 2009. published online 22 September 2009.

Abstract

In this study a set of 29 X-chromosomal short tandem repeats (STRs) located within the Xq26 region was evaluated. These STRs were found within the 133.14–133.45Mb region around the HPRTB locus. Evaluation of the microsatellites was performed with regard to polymorphism, reliable amplification, and low stutter artefacts. DXS10101, DXS10102, and DXS10103 were identified as those X-STRs with highest diversity; i.e. PIC values of 0.7174–0.8933. The locus DXS10101 was the optimal candidate for the integration in the commercial available test system Mentype Argus X-8 PCR amplification kit.