Forensic Science International: Genetics
Volume 4, Issue 3 , Pages 200-205 , April 2010

Casework testing of the multiplex kits AmpFℓSTR® SEfiler Plus™ PCR amplification kit (AB), PowerPlex® S5 System (Promega) and AmpFℓSTR® MiniFiler™ PCR amplification kit (AB)

  • Kathrin Müller

      Affiliations

    • Institute of Legal Medicine, University Hospital Ulm, Ulm, Germany
    • ,
  • Thomas Sommerer

      Affiliations

    • Institute of Legal Medicine, University Hospital Ulm, Ulm, Germany
    • ,
  • Erich Miltner

      Affiliations

    • Institute of Legal Medicine, University Hospital Ulm, Ulm, Germany
    • ,
  • Harald Schneider

      Affiliations

    • Landeskriminalamt Hessen, Hoelderlinstr. 5, D-65187 Wiesbaden, Germany
    • ,
  • Peter Wiegand

      Affiliations

    • Institute of Legal Medicine, University Hospital Ulm, Ulm, Germany
    • Corresponding Author InformationCorresponding author at: Institut für Rechtsmedizin, Universität Ulm, Albert-Einstein-Allee 11, D-89081 Ulm, Germany. Tel.: +49 731 500 65030; fax: +49 731 500 65040.

Received 12 March 2009 ,Revised 6 August 2009 ,Accepted 4 September 2009.

References 

  1. Wiegand P, Kleiber M. Less is more—length reduction of STR amplicons using redesigned primers. Int. J. Legal Med. 2001;114:285–287
  2. Wiegand P, Klein R, Braunschweiger G, Hohoff C, Brinkmann B. Short amplicon STR multiplex for stain typing. Int. J. Legal Med. 2006;120:160–164
  3. Butler JM, Shen Y, McCord BR. The development of reduced size STR amplicons as tools for analysis of degraded DNA. J. Forensic Sci. 2003;48:1054–1064
  4. Grubwieser P, Mühlmann R, Berger B, Niederstätter H, Pavlic M, Parson W. A new “miniSTR-multiplex” displaying reduced amplicon lengths for the analysis of degraded DNA. Int. J. Legal Med. 2006;120:115–120
  5. Chung DT, Drábek J, Opel KL, Butler JM, McCord BR. A study on the effects of degradation and template concentration on the amplification efficiency of the STR miniplex primer sets. J. Forensic Sci. 2004;49(4):733–740
  6. Coble MD, Butler JM. Characterization of new miniSTR loci to aid analysis of degraded DNA. J. Forensic Sci. 2005;50:43–53
  7. Müller K, Klein R, Miltner E, Wiegand P. Q8-A short amplicon multiplex including the German DNA database systems. Forensic Sci. Int. Genet. 2007;1(2):205–207
  8. Gill P, Fereday L, Morling N, Schneider PM. New multiplexes for Europe—amendments and clarification of strategic development. Forensic Sci. Int. 2006;163(1):155–157
  9. Gill P, Fereday L, Morling N, Schneider PM. The evolution of DNA databases—recommendations for new European STR loci. Forensic Sci. Int. 2006;156:242–244
  10. Mulero JJ, Chang CW, Lagace RE, Wang DY, Bas JL, McMahon TP, et al. Development and validation of the AmpFℓSTR® MiniFiler™ PCR amplification kit: a miniSTR multiplex for the analysis of degraded and/or PCR inhibited DNA. J Forensic Sci. 2008;53:838–852
  11. Junge A, Lederer T, Braunschweiger G, Madea B. Validation of the multiplex kit genRESMPX-2 for forensic casework analysis. Int. J. Legal Med. 2003;117:317–325
  12. Müller K, Klein R, Miltner E, Wiegand P. Improved STR typing of telogen hair root and hair shaft DNA. Electrophoresis. 2007;28:2835–2842
  13. Gill P. Application of low copy number DNA profiling. Croat. Med. J. 2001;42:229–232
  14. Kloostermann AD, Kersbergen P. Efficacy and limits of genotyping low copy number (LCN) DNA samples by multiplex PCR of STR loci. J. Soc. Biol. 2003;197:351–359
  15. Müller K, Klein R, Miltner E, Wiegand P. Q8-A short amplicon multiplex including the German DNA database systems. Forensic Sci. Int. 2007;1:205–207
  16. K. Müller, G. Braunschweiger, R. Klein, E. Miltner, P. Wiegand, Validation of the short amplicon multiplex Q8 including the German DNA database systems, J. Forensic Sci. (2009), in press
  17. Coticone SR, Oldroyd N, Philips H, Foxall P. Development of the AmpFℓSTR® SEfiler PCR amplification kit: a new multiplex containing the highly discriminating ACTBP2 (SE33) locus. Int. J. Legal Med. 2004;118:224–234

PII: S1872-4973(09)00145-8

doi: 10.1016/j.fsigen.2009.09.004

Forensic Science International: Genetics
Volume 4, Issue 3 , Pages 200-205 , April 2010

Article Tools

* Image Usage & Resolution