Forensic Science International: Genetics
Volume 4, Issue 4 , Pages 239-243 , July 2010

Validation of a dual cycle ethylene oxide treatment technique to remove DNA from consumables used in forensic laboratories

Received 24 June 2009 ,Revised 1 October 2009 ,Accepted 2 October 2009.

References 

  1. Gill P, Whittaker J, Flaxman C, Brown N, Buckleton J. An investigation of the rigor of interpretation rules for STRs derived from less than 100pg of DNA. Forensic Sci. Int. 2000;112:17–40
  2. Graham E. DNA reviews: low level DNA profiling. Forensic Sci. Med. Pathol. 2008;4:129–131
  3. Schmidt T, Hummel S, Herrmann B. Evidence of contamination in PCR laboratory disposables. Naturwissenschaften. 1995;82:423–431
  4. Sullivan K, Johnson P, Rowlands D, Allen H. New developments and challenges in the use of the UK DNA Database: addressing the issue of contaminated consumables. Forensic Sci. Int. 2004;146S:S175–S176
  5. Tamariz J, Kritina Voynarovska B, Prinz M, Caragine T. The application of ultraviolet irradiation to exogenous sources of DNA in plastic ware and water for the amplification of low copy number DNA. J. Forensic Sci. 2006;51:790–794
  6. Shaw K, Sesardic I, Bristol N, Ames C, Dagnall K, Ellis C, et al. Comparison of the effects of sterilisation techniques on subsequent DNA profiling. Int. J. Legal Med. 2008;122:29–33
  7. Altshuler M. PCR Troubleshooting: The Essential Guide. Norfolk, UK: Caister Academic Press; 2006;
  8. Lawley P, Brookes P. Further studies on the alkylation of nucleic acids and their constituent nucleotides. Biochem. J. 1963;89:127
  9. Nygren J, Cedervall B, Eriksson S, Duškinská M, Kolman A. Induction of DNA strand breaks by ethylene oxide in human diploid fibroblasts. Environ. Mol. Mutagenesis. 1994;24:161–167
  10. Lowe A, Murray C, Whittaker J, Tully G, Gill P. The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces. Forensic Sci. Int. 2002;129:25–34
  11. Lien K, Liu C, Kuo P, Lee G. Microfluidic system for detection of Thalassemia-1 deletion using saliva samples. Anal. Chem. 2009;81:4502–4509
  12. Rutty G, Hopwood A, Tucker V. The effectiveness of protective clothing in the reduction of potential DNA contamination of the scene of crime. Int. J. Legal Med. 2003;117:170–174
  13. Gill P. Application of low copy number DNA profiling. Croat. Med. J. 2001;42:229–232
  14. Howitt T. Ensuring the integrity of results: a continuing challenge in forensic DNA analysis. In: Promega Genetic Identity Conference Proceedings Fourteenth International Symposium on Human Identification. 2003;

PII: S1872-4973(09)00153-7

doi: 10.1016/j.fsigen.2009.10.004

Forensic Science International: Genetics
Volume 4, Issue 4 , Pages 239-243 , July 2010