Forensic Science International: Genetics
Volume 4, Issue 4 , Pages 244-256 , July 2010

The development of a mRNA multiplex RT-PCR assay for the definitive identification of body fluids

Received 2 July 2009 ,Revised 10 September 2009 ,Accepted 2 October 2009.

References 

  1. Tobe SS, Watson N, Daéid NN. Evaluation of six presumptive tests for blood, their specificity, sensitivity, and effect on high molecular-weight DNA. J. Forensic Sci. 2007;52:102–109
  2. Higaki RS, Philip WMS. A study of the sensitivity, stability and specificity of phenolphthalein as an indicator test for blood. Can. Soc. Forensic Sci. J. 1996;97–102
  3. Ponce AC, Pascual FAV. Critical revision of presumptive tests for bloodstains. Forensic Sci. Commun. 1999;1:1–15
  4. Gaensslen RE. Sourcebook in Forensic Serology, Immunology, and Biochemistry. Washington, DC: U.S. Department of Justice; 1983;
  5. Willott GM, Griffiths M. A new method for locating saliva stains – spotty paper for spotting spit. Forensic Sci. Int. 1980;15:79–83
  6. Hedman J, Gustavsson K, Ansell R. Using the new Phadebas® forensic press test to find crime scene saliva stains suitable for DNA analysis. Forensic Sci. Int. Genet. Supp. Ser. 2008;1:430–432
  7. Mayers JR, Adkins WK. Comparison of modern techniques for saliva screening. J. Forensic Sci. 2008;53:862–867
  8. Hochmeister MN, Budowle B, Rudin O, Gehrig C, Borer U, Thali M, et al. Evaluation of prostate-specific antigen (PSA) membrane test assays for the forensic identification of seminal fluid. J. Forensic Sci. 1999;44:1057–1060
  9. Sato I, Sagi M, Ishiwari A, Nishijima H, Ito HE, Mukai T. Use of the “SMITEST” PSA card to identify the presence of prostate-specific antigen in semen and male urine. Forensic Sci. Int. 2002;127:71–74
  10. Setzer M, Juusola J, Ballantyne J. Recovery and stability of RNA in vaginal swabs and blood, semen, and saliva stains. J. Forensic Sci. 2008;53:296–305
  11. Zubakov D, Hanekamp E, Kokshoorn M, van Ijeken W, Kayser M. Stable RNA markers for identification of blood and saliva stains revealed from whole genome expression analysis of time-wise degraded samples. Int. J. Legal Med. 2008;122:135–142
  12. Bauer M, Patzelt D. Evaluation of mRNA markers for the identification of menstrual blood. J. Forensic Sci. 2002;47:1–5
  13. Bauer M, Patzelt D. A method for simultaneous RNA and DNA isolation from dried blood and semen stains. Forensic Sci. Int. 2003;136:76–78
  14. Juusola J, Ballantyne J. Messenger RNA profiling: a prototype method to supplant conventional methods for body fluid identification. Forensic Sci. Int. 2003;135:85–96
  15. Alvarez M, Juusola J, Ballantyne J. An mRNA and DNA co-isolation method for forensic casework samples. Anal. Biochem. 2004;335:289–298
  16. Juusola J, Ballantyne J. Multiplex mRNA profiling for the identification of body fluids. Forensic Sci. Int. 2005;152:1–12
  17. Fang R, Manohar CF, Shulse C, Brevnov M, Wong A, Petrauskene OV, et al. Real-time PCR assays for the detection of tissue and body fluid specific mRNAs. Int. Cong. Ser. 2006;1288:685–687
  18. Nussbaumer C, Gharehbaghi-Schnell E, Korschineck I. Messenger RNA profiling: a novel method for body fluid identification by real-time PCR. Forensic Sci. Int. 2006;157:181–186
  19. Juusola J, Ballantyne J. mRNA profiling for body fluid identification by multiplex quantitative RT-PCR. J. Forensic Sci. 2007;52:1–11
  20. Haas C, Klesser B, Maake C, Bär W, Kratzer A. mRNA profiling for body fluid identification by reverse transcription endpoint PCR and real-time PCR. Forensic Sci. Int. Genet. 2009;3:80–88
  21. Hadley TJ. Invasion of erythrocytes by malaria parasites: a cellular and molecular overview. Ann. Rev. Microbiol. 1986;40:451–477
  22. Independent Forensics (2008) http://www.ifi-test.com/index.php.
  23. Hulboy DL, Rudoph LA, Matrisian LM. Matrix metalloproteinases as mediators of reproductive function. Mol. Hum. Reprod. 1997;3(1):27–45
  24. Ferri G, Bini C, Ceccardi S, Pelotti S. Successful identification of two years old menstrual bloodstain by using MMP-11 shorter amplicons. J. Forensic Sci. 2004;49:1387
  25. Steger K, Pauls K, Klonisch T, Franke FE, Bergmann FEM. Expression of protamine-1 and -2 mRNA during human spermatogenesis. Mol. Hum. Reprod. 2000;6:219–225
  26. Wykes SM, Visscher DW, Krawetz SA, Haploid SA. Transcripts persist in mature human spermatozoa. Mol. Hum. Reprod. 1997;3:15–19
  27. Beninati S, Piacentini M. The transglutaminase family: an overview: minireview article. Amino Acids. 2004;26:367–372
  28. Dubbink HJ, de Waal L, van Haperen R, Verkaik NS, Trapman J, Romijn JC. The human prostate-specific transglutaminase gene (tgm4): genomic organization, tissue-specific expression, and promoter characterization. Gene. 1998;51:434–444
  29. Schenkels L, Veerman E, Amerongen A. Biochemical composition of human saliva in relation to other mucosal fluids. Crit. Rev. Oral Biol. Med. 1995;6:161–175
  30. Li Y, Joh MARSt, Zhou X, Kim Y, Sinha U, Jordan R, et al. Salivary transcriptome diagnostics for oral cancer detection. Clin. Cancer Res. 2004;10:8442–8450
  31. Park NJ, Li Y, Yu T, Brinkman BMN, Wong DT. Characterization of RNA in saliva. Clin. Chem. 2006;52:988–994
  32. Park NJ, Zhou X, Yu T, Brinkman BMN, Zimmermann BG, Palanisamy V, et al. Characterization of salivary RNA by cDNA library analysis. Arch. Oral Biol. 2007;52:30–35
  33. Barratt CLR, Kay V, Oxenham SK. The human spermatozoon- a stripped down but refined machine. J. Biol. 2009;8:63–65

PII: S1872-4973(09)00155-0

doi: 10.1016/j.fsigen.2009.10.006

Forensic Science International: Genetics
Volume 4, Issue 4 , Pages 244-256 , July 2010