Forensic Science International: Genetics
Volume 4, Issue 4 , Pages 257-264 , July 2010

Developmental validation of the PowerPlex® 16 HS System: An improved 16-locus fluorescent STR multiplex

  • Martin G. Ensenberger

      Affiliations

    • Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, United States
    • Corresponding Author InformationCorresponding author at: Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, United States. Tel.: +1 800 356 9526x4848; fax: +1 608 298 4808.
    • ,
  • Jonelle Thompson

      Affiliations

    • Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, United States
    • ,
  • Becky Hill

      Affiliations

    • National Institute of Standards and Technology, 100 Bureau Dr., M/S 8312, Gaithersburg, MD 20899, United States
    • ,
  • Kristen Homick

      Affiliations

    • Erie County Central Police Services Forensic Laboratory, 45 Elm St., Buffalo, NY 14203-2600, United States
    • ,
  • Veronica Kearney

      Affiliations

    • Tucson Police Department, 270 S Stone Ave., Tucson, AZ 85701-1917, United States
    • ,
  • Kathleen A. Mayntz-Press

      Affiliations

    • Arizona Department of Public Safety, 2102 W. Encanto Blvd., Phoenix, AZ 85009, United States
    • ,
  • Paul Mazur

      Affiliations

    • Erie County Central Police Services Forensic Laboratory, 45 Elm St., Buffalo, NY 14203-2600, United States
    • ,
  • Amy McGuckian

      Affiliations

    • Palm Beach County Sheriff's Office, 3228 Gun Club Rd., West Palm Beach, FL 33406, United States
    • ,
  • Jelena Myers

      Affiliations

    • Tucson Police Department, 270 S Stone Ave., Tucson, AZ 85701-1917, United States
    • ,
  • Kelli Raley

      Affiliations

    • Arizona Department of Public Safety, 2102 W. Encanto Blvd., Phoenix, AZ 85009, United States
    • ,
  • Stewart G. Raley

      Affiliations

    • Arizona Department of Public Safety, 2102 W. Encanto Blvd., Phoenix, AZ 85009, United States
    • ,
  • Robin Rothove

      Affiliations

    • Missouri State Highway Patrol Crime Laboratory Division, 1510 E. Elm St., Jefferson City, MO 65101, United States
    • ,
  • Jonathan Wilson

      Affiliations

    • Tulsa Police Department Forensic Laboratory, 600 Civic Center Dr., Tulsa, OK 74103-3822, United States
    • ,
  • Doug Wieczorek

      Affiliations

    • Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, United States
    • ,
  • Patricia M. Fulmer

      Affiliations

    • Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, United States
    • ,
  • Douglas R. Storts

      Affiliations

    • Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, United States
    • ,
  • Benjamin E. Krenke

      Affiliations

    • Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, United States

Received 4 August 2009 ,Revised 15 September 2009 ,Accepted 2 October 2009.

References 

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  2. Moretti T. The CODIS STR Project: evaluation of fluorescent multiplex STR systems. In: Presented at the American Academy of Forensic Sciences Annual Meeting. February 9–14; San Francisco, CA. 1998;
  3. Budowle B. Examples of STR population databases for CODIS and for casework. In: Presented at the 9th International Symposium on Human Identification. October 7–10; Orlando, FL. 1998;
  4. Sprecher C, Krenke B, Amiott B, Rabbach D, Grooms K. The PowerPlex™ System. Profiles DNA. 2000;4:3–6
  5. Krenke BE, Tereba A, Anderson SJ, Buel E, Culhane S, Finis CJ, et al. Validation of a 16-Locus fluorescent multiplex system. J. Forensic Sci. 2002;47:773–785
  6. Levadokou E, Freeman DA, Budzynski MJ, Early BE, McElfresh KC, Schumm JW, et al. Allele frequencies for fourteen STR loci of the PowerPlex® 1.1 and 2.1 multiplex systems and Penta D locus in Caucasians, African-Americans, Hispanics and other populations of the United States of America and Brazil. J. Forensic Sci. 2001;46:736–761
  7. Bacher J, Schumm JW. Development of highly polymorphic pentanucleotide tandem repeat loci with low stutter. Profiles in DNA. 1998;2:3–6
  8. Bacher JW, Helms C, Donis-Keller H, Hennes L, Nassif N, Schumm JW. Chromosome localization of CODIS loci and new pentanucleotide repeat loci. In: Proceedings of the 18th International ISFH Congress. 1999;p. 33–36
  9. Budowle B, Sprecher C. Concordance study on population database samples using the PowerPlex® 16 and AmpflSTR® Profiler Plus™ and AmpflSTR® COfiler™ Kit. J. Forensic Sci. 2001;46:637–641
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  17. Akane A, Matsubara K, Nakamura H, Takahashi S, Kimura K. Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification. J. Forensic Sci. 1994;39:362–372
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  19. Ensenberger MG, Fulmer PM. The PowerPlex® 16 HS System. Profiles in DNA. 2009;12:9–11
  20. McLaren R. PowerPlex® 16 versus Identifiler® Systems—sensitivity and effects of inhibitors. Promega Corporation Application Note #AN156. Madison, WI: Promega Corporation; 2007;
  21. McLaren RS, Ensenberger MG, Budowle B, Rabbach D, Fulmer PM, Sprecher CJ, et al. Post-injection hybridization of complementary DNA strands on capillary electrophoresis platforms: a novel solution for dsDNA artifacts. Forensic Sci. Int. Genet. 2008;2:257–273
  22. Gill P. Application of low copy number DNA profiling. Croat. Med. J. 2001;42:229–232
  23. Moretti TR, Baumstark AL, Defenbaugh DA, Keys KM, Smerick JB, Budowle B. Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. J. Forensic Sci. 2001;46:647–660
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  27. Promega Corporation Part Number #GE053-Internal Validation of STR Systems. Madison, WI: Promega Corporation; 2006;

PII: S1872-4973(09)00156-2

doi: 10.1016/j.fsigen.2009.10.007

Forensic Science International: Genetics
Volume 4, Issue 4 , Pages 257-264 , July 2010

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