Forensic Science International: Genetics
Volume 4, Issue 5 , Pages 275-280 , October 2010

Simple and highly effective DNA extraction methods from old skeletal remains using silica columns

  • Hwan Young Lee

      Affiliations

    • Department of Forensic Medicine and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Republic of Korea
    • Human Identification Research Center, Yonsei University, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Republic of Korea
    • ,
  • Myung Jin Park

      Affiliations

    • Department of Forensic Medicine and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Republic of Korea
    • ,
  • Na Young Kim

      Affiliations

    • Department of Forensic Medicine and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Republic of Korea
    • ,
  • Jeong Eun Sim

      Affiliations

    • Department of Forensic Medicine and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Republic of Korea
    • ,
  • Woo Ick Yang

      Affiliations

    • Department of Forensic Medicine and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Republic of Korea
    • ,
  • Kyoung-Jin Shin

      Affiliations

    • Department of Forensic Medicine and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Republic of Korea
    • Human Identification Research Center, Yonsei University, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Republic of Korea
    • Corresponding Author InformationCorresponding author at: Department of Forensic Medicine, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-Gu, Seoul 120-752, Republic of Korea. Tel.: +82 2 2228 2481; fax: +82 2 362 0860.

Received 1 June 2009 ,Revised 9 October 2009 ,Accepted 10 October 2009.

References 

  1. Cooper A, Poinar HN. Ancient DNA: do it right or not at all. Science. 2000;289:1139
  2. Hofreiter M, Serre D, Poinar HN, Kuch M, Pääbo S. Ancient DNA. Nat. Rev. Genet. 2001;2:353–359
  3. Pääbo S, Poinar H, Serre D, Jaenicke-Despres V, Hebler J, Rohland N, et al. Genetic analyses from ancient DNA. Annu. Rev. Genet. 2004;38:645–679
  4. Willerslev E, Cooper A. Ancient DNA. Proc. Biol. Sci. 2005;272:3–16
  5. Höss M, Pääbo S. DNA extraction from Pleistocene bones by a silica-based purification method. Nucleic Acids Res. 1993;21:3913–3914
  6. Hänni C, Brousseau T, Laudet V, Stehelin D. Isopropanol precipitation removes PCR inhibitors from ancient bone extracts. Nucleic Acids Res. 1995;23:881–882
  7. Yang DY, Eng B, Waye JS, Dudar JC, Saunders SR. Technical note: improved DNA extraction from ancient bones using silica-based spin columns. Am. J. Phys. Anthropol. 1998;105:539–543
  8. Kalmar T, Bachrati CZ, Marcsik A, Rasko I. A simple and efficient method for PCR amplifiable DNA extraction from ancient bones. Nucleic Acids Res. 2000;28:E67
  9. Leonard JA, Wayne RK, Cooper A. Population genetics of ice age brown bears. Proc. Natl. Acad. Sci. U.S.A. 2000;97:1651–1654
  10. Baker LE, McCormick WF, Matteson KJ. A silica-based mitochondrial DNA extraction method applied to forensic hair shafts and teeth. J. Forensic Sci. 2001;46:126–130
  11. Hofreiter M, Rabeder G, Jaenicke-Després V, Withalm G, Nagel D, Paunovic M, et al. Evidence for reproductive isolation between cave bear populations. Curr. Biol. 2004;14:40–43
  12. Loreille OM, Diegoli TM, Irwin JA, Coble MD, Parsons TJ. High efficiency DNA extraction from bone by total demineralization. Forensic Sci. Int. Genet. 2007;1:191–195
  13. Davoren J, Vanek D, Konjhodzić R, Crews J, Huffine E, Parsons TJ. Highly effective DNA extraction method for nuclear short tandem repeat testing of skeletal remains from mass graves. Croat. Med. J. 2007;48:478–485
  14. Rohland N, Hofreiter M. Comparison and optimization of ancient DNA extraction. BioTechniques. 2007;42:343–352
  15. Westring CG, Kristinsson R, Gilbert DM, Danielson PB. Validation of reduced-scale reactions for the Quantifiler Human DNA kit. J. Forensic Sci. 2007;52:1035–1043
  16. Gill P, Whitaker J, Flaxman C, Brown N, Buckleton J. An investigation of the rigor of interpretation rules for STRs derived from less than 100pg of DNA. Forensic Sci. Int. 2000;112:17–40
  17. Tian H, Hühmer AF, Landers JP. Evaluation of silica resins for direct and efficient extraction of DNA from complex biological matrices in a miniaturized format. Anal. Biochem. 2000;283:175–191

PII: S1872-4973(10)00005-0

doi: 10.1016/j.fsigen.2009.10.014

Forensic Science International: Genetics
Volume 4, Issue 5 , Pages 275-280 , October 2010

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