Forensic Science International: Genetics
Volume 4, Issue 5 , Pages e145-e148, October 2010

Analysis of 14 highly informative SNP markers on X chromosome by TaqMan® SNP genotyping assay

  • Li Li

      Affiliations

    • Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China
    • Corresponding Author InformationCorresponding author. Tel.: +86 021 52352959; fax: +86 02153352959.
    • ,
  • Chengtao Li

      Affiliations

    • Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China
    • ,
  • Suhua Zhang

      Affiliations

    • Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China
    • Medical School of Soochow University, Suzhou 215123, PR China
    • ,
  • Shumin Zhao

      Affiliations

    • Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China
    • ,
  • Yan Liu

      Affiliations

    • Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China
    • ,
  • Yuan Lin

      Affiliations

    • Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China

Received 27 August 2009; received in revised form 26 January 2010; accepted 17 April 2010. published online 21 May 2010.

Abstract 

Objective

To genotype X-linked single nucleotide polymorphisms by TaqMan® SNP genotyping assay.

Methods

14 SNP markers on X chromosome were selected and genotyped by TaqMan® SNP genotyping assay in Chinese Han population samples.

Results

According to the results of population studies, deviations from Hardy–Weinberg equilibrium could be found at rs1299087 SNP loci, no deviations from Hardy–Weinberg equilibrium could be found at the other 13 SNP loci. The 14 X-SNPs were high informative. The overall power of discrimination (CPD) in females and in males were 0.999998379 and 0.999899, respectively. The combined exclusion probability in paternity testing for trios and duos were 0.9983 and 0.9788, respectively.

Conclusion

SNP typing by TaqMan® SNP genotyping assay is suitable for low-throughput application. The forensic efficiency parameters showed that the 14 X-linked SNPs are highly informative and seem to be useful for forensic genetics except rs1299087.

Keywords: Forensic genetics, Genotyping, SNP, TaqMan® SNP genotyping assay, X chromosome, Population data

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PII: S1872-4973(10)00078-5

doi:10.1016/j.fsigen.2010.04.004

Forensic Science International: Genetics
Volume 4, Issue 5 , Pages e145-e148, October 2010

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