Forensic Science International: Genetics
Volume 5, Issue 1 , Pages 69-73 , January 2011

The use of fluorescence in situ hybridisation and laser microdissection to identify and isolate male cells in an azoospermic sexual assault case

Received 16 April 2009 ,Revised 1 April 2010 ,Accepted 28 April 2010.

References 

  1. Yu H, Berkel H. Prostate specific antigen (PSA) in women. J. La State Med. Soc. 1999;Vol.151:
  2. Lunetta P, Sippel H. Positive prostate-specific antigen (PSA) reaction in post-mortem rectal swabs: a cautionary note. J. Forensic Legal Med. 2009;16:397–399
  3. Shaler RC, Ryan P. High acid phosphatase levels as a possible false indicator of the presence of seminal fluid. Am. J. Forensic Med. Pathol. 1982;3(2):
  4. Virkler K, Lednev IK. Raman spectroscopic signature of semen and its potential application to forensic body fluid identification. Forensic Sci. Int. 2009;192:56–62
  5. Haas C, Munheim C, Kratzer A, Bär W, Maake C. mRNA profiling for the identification of sperm and seminal plasma. Forensic Sci. Int.: Genet. Suppl. Series. 2009;2:534–535
  6. Gill P, Jeffreys AJ, Werrett DJ. Forensic applications of DNA “fingerprints”. Nature. 1985;318:577–579
  7. Willott GM. Frequency of Azoospermia. Forensic Sci. Int. 1982;20:9–10
  8. Pollak OJ. Semen and seminal stains. Arch. Pathol. 1943;35:140–196
  9. Murray C, McAlister C, Elliott K. Identification and isolation of male cells using fluorescence in situ hybridisation and laser microdissection, for use in the investigation of sexual assault. Forensic Sci. Int.: Genet. 2007;1:247–252
  10. Collins KA, Rao PN, Hayworth R, Schnell S, Tap MP, Lantz PE, et al. Identification of sperm and non-sperm male cells in cervicovaginal smears using fluorescence in situ hybridisation: applications in alleged sexual assault cases. J. Forensic Sci. 1994;39(6):1347–1355
  11. Dziegelewski M, Simich JP, Rittenhouse-Olson K. Use of a Y chromosome probe as an aid in the forensic proof of sexual assault. J. Forensic Sci. 2002;47(3):601–604
  12. Rao PN, Collins KA, Geisinger KR, Parsons LH, Schnell S, Hayworth-Hodge R, et al. Identification of male epithelial cells in routine postcoital cervicovaginal smears using fluorescence in situ hybridization: application in sexual assault and molestation. Am. J. Clin. Pathol. 1995;104(1):32–35
  13. Prahlow JA, Lantz PE, Cox-Jones K, Rao PN, Pettenati MJ. Gender identification of human hair using fluorescence in situ hybridization. J. Forensic Sci. 1996;41(6):1035–1037
  14. Collins KA, Cina SJ, Pettenati MJ, Fitts M. Identification of female cells in postcoital penile swabs using fluorescence in situ hybridization. Arch. Pathol. Lab. Med. 2000;124(7):1080–1082
  15. Cina SJ, Collins KA, Fitts M, Pettenati MJ. Isolation and Identification of male and female DNA on a postcoital condom. Arch. Pathol. Lab. Med. 2000;124(7):1083–1086
  16. Di Martino D, Giuffre G, Staiti N, Simone A, Le Donne M, Savaro L. Single sperm cell isolation by laser microdissection. Forensic Sci. Int. 2004;146S:S151–S153
  17. Sanders CT, Sanchez N, Ballantyne J, Peterson DA. Laser microdissection separation of pure spermatozoa from epithelial cells for short tandem repeat analysis. J. Forensic Sci. 2006;51(4):748–757
  18. Anslinger K, Mack B, Bayer B, Rolf B, Eisenmenger W. Digoxigenin labelling and laser capture microdissection of male cells. Int. J. Legal Med. 2005;119(6):374–377
  19. Anslinger K, Bayer B, Mack B, Eisenmenger W. Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling. Int. J. Legal Med. 2007;121:54–56
  20. Gill P, Whitaker J, Flaxman C, Brown N, Buckleton J. An investigation of the rigor of interpretation rules for STRs derived from less than 100pg of DNA. Forensic Sci. Int. 2000;112:17–40
  21. Caddy B, Taylor GR, Linacre AMT. A Review of the Science of Low Template DNA Analysis. 2008;[online] Available at: http://police.homeoffice.gov.uk/publications/operationalpolicing/Review_of_Low_Template_DNA_1.html

PII: S1872-4973(10)00082-7

doi: 10.1016/j.fsigen.2010.04.008

Forensic Science International: Genetics
Volume 5, Issue 1 , Pages 69-73 , January 2011

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