Genetic analysis of 15 autosomal and 12 Y-STR loci in the Espirito Santo State population, Brazil

Wolfgramm, Eldamária de Vargas; Silva, Beatriz Candida; da Costa Aguiar, Vitor Resende; Malta, Frederico Scott Varela; de Castro, Amanda Mafia; de Souza Ferreira, Alessandro Clayton; Prezoti, Alessandra Nunes Loureiro; de Paula, Flavia; Louro, Iúri Drumond

doi:10.1016/j.fsigen.2010.05.001

« Previous Next »Forensic Science International: Genetics
Volume 5, Issue 3 , Pages e41-e43, June 2011

Genetic analysis of 15 autosomal and 12 Y-STR loci in the Espirito Santo State population, Brazil

Eldamária de Vargas Wolfgramm
- Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, CEP 29040-090 Vitória, ES, Brazil
- These authors contributed equally for this manuscript.
- Tel.: +55 27 3335 7251; fax: +55 27 3335 7250.
Beatriz Candida Silva
- Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, CEP 29040-090 Vitória, ES, Brazil
- These authors contributed equally for this manuscript.
- Tel.: +55 27 3335 7251; fax: +55 27 3335 7250.
Vitor Resende da Costa Aguiar
- Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, CEP 29040-090 Vitória, ES, Brazil
- Tel.: +55 27 3335 7251; fax: +55 27 3335 7250.
Frederico Scott Varela Malta
- Instituto Hermes Pardini, Departamento de Genética Humana, Rua Maranhão, 1040 Funcionários, 30150-3330 Belo Horizonte, MG, Brazil
- Tel.: +55 31 3228 3205; fax: +55 31 3228 3205.
Amanda Mafia de Castro
- Instituto Hermes Pardini, Departamento de Genética Humana, Rua Maranhão, 1040 Funcionários, 30150-3330 Belo Horizonte, MG, Brazil
- Tel.: +55 31 3228 3205; fax: +55 31 3228 3205.
Alessandro Clayton de Souza Ferreira
- Instituto Hermes Pardini, Departamento de Genética Humana, Rua Maranhão, 1040 Funcionários, 30150-3330 Belo Horizonte, MG, Brazil
- Tel.: +55 31 3228 3205; fax: +55 31 3228 3205.
Alessandra Nunes Loureiro Prezoti
- Centro de Hematologia e Hemoterapia do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil
- Tel.: +55 27 3137 2466; fax: +55 27 3137 2463.
Flavia de Paula
- Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, CEP 29040-090 Vitória, ES, Brazil
- Tel.: +55 27 3335 7251; fax: +55 27 3335 7250.
Iúri Drumond Louro
- Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, CEP 29040-090 Vitória, ES, Brazil
- Corresponding author. Tel.: +55 27 3335 7251; fax: +55 27 3335 7250.

Received 10 April 2009; received in revised form 25 April 2010; accepted 4 May 2010. published online 07 June 2010.

Abstract

This study provides population genetic data for individuals of Vitoria, Espirito Santo, Brazil, a location not yet characterized for STR frequencies used for genetic identification studies. Allelic frequencies and other population data analysis are reported for the 15 autosomal-STR loci included in the PowerPlex^®16 kit (CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, Penta D, Penta E, TPOX, TH01 and vWA). Allele and haplotype frequencies, gene diversity and discrimination capacity were also estimated for the PowerPlex^® Y System (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Blood samples were obtained from 226 unrelated volunteers (135 males and 91 females) residents in the city of Vitoria, representing a typical sample of the mixed ethnicity present in the Espirito Santo State, Brazil. Within the tested population, the total number of individuals typed for specific markers is: 226 for D13S317, D21S11, D3S1358, D7S820, D8S1179 and FGA; 225 for D16S539 and D5S818; 224 for D18S51; 223 for CSF1PO; 222 for Penta D and vWA; 220 for Penta E; 207 for TPOX and 142 for TH01. Y-STR haplotypes were analyzed for 102 unrelated males, being 71 of them present in the 135 autosomal-STR sample, and 31 new males tested only for Y-STR markers. All autosomal markers were in Hardy–Weinberg Equilibrium. Y-STR analysis identified 101 haplotypes, being 100 of them unique.

Keywords: Autosomal STRs, Y-chromosome STRs, Haplotypes, Allelic frequencies, Population data, Vitoria, Espirito Santo, Brazil