Population data and sequence analysis of a ‘new’ microsatellite locus HumHUU (D16S3433)

Konarzewska, Magdalena; Pepinski, Witold; Jagiello, A.; Spolnicka, M.; Soltyszewski, I.; Plucienniczak, A.; Berent, J.

doi:10.1016/j.fsigen.2010.03.018

« Previous Next »Forensic Science International: Genetics
Volume 4, Issue 5 , Pages e143-e144, October 2010

Population data and sequence analysis of a ‘new’ microsatellite locus HumHUU (D16S3433)

Magdalena Konarzewska
- Department of Medical Genetics, Warsaw Medical University, Warsaw, Poland
Witold Pepinski
- Department of Forensic Medicine, Medical University of Bialystok, Bialystok, Poland
- Corresponding author. Tel.: +48 85 7485948; fax: +48 85 7485948.
A. Jagiello
- Institute of Biotechnology and Antibiotics, Warsaw, Poland
M. Spolnicka
- Department of Biology, Central Forensic Laboratory, General Headquarters of Police, Warsaw, Poland
I. Soltyszewski
- Department of Criminalistics and Forensic Medicine, University of Warmia and Mazury, Olsztyn, Poland
A. Plucienniczak
- Institute of Biotechnology and Antibiotics, Warsaw, Poland
J. Berent
- Department of Forensic Medicine, Medical University of Lodz, Lodz, Poland

Received 4 October 2009; received in revised form 10 February 2010; accepted 12 March 2010. published online 16 June 2010.

Abstract

This study reports sequence characteristics and population genetic data on a ‘new’ STR locus HumHUU (D16S3433) located in the non-coding region of chromosome 16q. Based on a population sample of 306 non-related Polish individuals 205 genotypes and 15 alleles with length range of 157–211bp were distinguished. No deviation from HWE was observed. The sequence analysis of each D16S3433 allele revealed a tetranucleotide repeat motif with a basic sequence structure (AAAA)_0–1(AAAG)_11–22(AAAAG)(AAAA)(AG)(AAAAAAG). The power of discrimination is 0.9538, showing a high degree of polymorphism. The presented results demonstrate that the D16S3433 is a useful genetic marker for forensic purposes and paternity testing.