Forensic Science International: Genetics
Volume 5, Issue 5 , Pages 369-375 , November 2011

Autosomal SNP typing of forensic samples with the GenPlex™ HID System: Results of a collaborative study

  • C. Tomas

      Affiliations

    • Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, 11 Frederik V's Vej, DK-2100 Copenhagen, Denmark1
    • Corresponding Author InformationCorresponding author. Tel.: +45 35326283; fax: +45 35326270.
    • ,
  • G. Axler-DiPerte

      Affiliations

    • New York City Office of Chief Medical Examiner, Department of Forensic Biology New York, NY, USA
    • ,
  • Z.M. Budimlija

      Affiliations

    • New York City Office of Chief Medical Examiner, Department of Forensic Biology New York, NY, USA
    • ,
  • C. Børsting

      Affiliations

    • Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, 11 Frederik V's Vej, DK-2100 Copenhagen, Denmark1
    • ,
  • M.D. Coble

      Affiliations

    • Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, MD, USA
    • ,
  • A.E. Decker

      Affiliations

    • U.S. National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD, USA
    • ,
  • A. Eisenberg

      Affiliations

    • University of North Texas Health Science Center, Institute of Investigative Genetics, University of North Texas for Human Identification, Fort Worth, TX, USA
    • ,
  • R. Fang

      Affiliations

    • Applied Biosystems/Life Technologies, Foster City, CA, USA
    • ,
  • M. Fondevila

      Affiliations

    • Institute of Legal Medicine, Genomic Medicine Group, University of Santiago de Compostela, Spain
    • ,
  • S. Frisk Fredslund

      Affiliations

    • Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, 11 Frederik V's Vej, DK-2100 Copenhagen, Denmark1
    • ,
  • S. Gonzalez

      Affiliations

    • University of North Texas Health Science Center, Institute of Investigative Genetics, University of North Texas for Human Identification, Fort Worth, TX, USA
    • ,
  • A.J. Hansen

      Affiliations

    • Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, 11 Frederik V's Vej, DK-2100 Copenhagen, Denmark1
    • ,
  • P. Hoff-Olsen

      Affiliations

    • Institute of Forensic Medicine, University of Oslo, Rikshospitalet, Norway
    • ,
  • C. Haas

      Affiliations

    • Institute of Legal Medicine, University of Zurich, Switzerland
    • ,
  • P. Kohler

      Affiliations

    • Institute of Forensic Medicine, University of Oslo, Rikshospitalet, Norway
    • ,
  • A.K. Kriegel

      Affiliations

    • Institute of Legal Medicine, University Hospital, Cologne, Germany
    • ,
  • B. Lindblom

      Affiliations

    • Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Linköping, Sweden
    • ,
  • F. Manohar

      Affiliations

    • Applied Biosystems/Life Technologies, Foster City, CA, USA
    • ,
  • O. Maroñas

      Affiliations

    • Institute of Legal Medicine, Genomic Medicine Group, University of Santiago de Compostela, Spain
    • ,
  • H.S. Mogensen

      Affiliations

    • Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, 11 Frederik V's Vej, DK-2100 Copenhagen, Denmark1
    • ,
  • K. Neureuther

      Affiliations

    • Federal Criminal Police Office, Wiesbaden, Germany
    • ,
  • H. Nilsson

      Affiliations

    • Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Linköping, Sweden
    • ,
  • M.K. Scheible

      Affiliations

    • Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, MD, USA
    • ,
  • P.M. Schneider

      Affiliations

    • Institute of Legal Medicine, University Hospital, Cologne, Germany
    • ,
  • M.L. Sonntag

      Affiliations

    • Federal Criminal Police Office, Wiesbaden, Germany
    • ,
  • M. Stangegaard

      Affiliations

    • Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, 11 Frederik V's Vej, DK-2100 Copenhagen, Denmark1
    • ,
  • D. Syndercombe-Court

      Affiliations

    • Academic Haematology Unit, BICMS Pathology Group, Barts and The London School of Medicine and Dentistry, London, UK
    • ,
  • C.R. Thacker

      Affiliations

    • Academic Haematology Unit, BICMS Pathology Group, Barts and The London School of Medicine and Dentistry, London, UK
    • ,
  • P.M. Vallone

      Affiliations

    • U.S. National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD, USA
    • ,
  • A.A. Westen

      Affiliations

    • Department of Human Biological Traces (R&D), Netherlands Forensic Institute, The Hague, The Netherlands
    • ,
  • N. Morling

      Affiliations

    • Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, 11 Frederik V's Vej, DK-2100 Copenhagen, Denmark1

Received 17 May 2010 ,Revised 24 June 2010 ,Accepted 24 June 2010.

References 

  1. Sanchez JJ, Phillips C, Børsting C, Balogh K, Bogus M, Fondevila M, et al. A multiplex assay with 52 single nucleotide polymorphisms for human identification. Electrophoresis. 2006;27:1713–1724
  2. Sanchez JJ, Børsting C, Balogh K, Berger B, Bogus M, Butler JM, et al. Forensic typing of autosomal SNPs with a 29 SNP-multiplex – results of a collaborative EDNAP exercise. Forensic Sci. Int. Genet. 2008;2:176–183
  3. Børsting C, Sanchez JJ, Hansen HE, Hansen AJ, Bruun HQ, Morling N. Performance of the SNPforID 52-plex assay in paternity testing. Forensic Sci. Int. Genet. 2008;2:292–300
  4. Musgrave-Brown E, Ballard D, Balogh K, Bender K, Berger B, Bogus M, et al. Forensic validation of the SNPforID 52-plex assay. Forensic Sci. Int. Genet. 2007;1:186–190
  5. The SNPforID consortium, http://www.snpforid.org.
  6. Mengel-Jørgensen J, Sanchez JJ, Børsting C, Kirpekar F, Morling N. Typing of multiple SNPs using ribonuclease cleavage of DNA/RNA chimeric single base extension primers and detection by MALDI-TOF mass spectrometry. Anal. Chem. 2005;77:5229–5235
  7. Børsting C, Sanchez JJ, Morling N. SNP typing on the NanoChip electronic microarray. Methods Mol. Biol. 2005;297:155–168
  8. Harrison C, Musgrave-Brown E, Bender K, Carracedo A, Morling N, Schneider P, et al. A sensitive issue: pyrosequencing as a valuable forensic SNP typing platform. Int. Congr. Ser. 2006;1288:52–54
  9. Sobrino B, Lareu M, Brion M, Carracedo A. SNP genotyping with single base extension-tag microarrays. Int. Congr. Ser. 2004;1261:331–333
  10. Krjutškov K, Viltrop T, Palta P, Metspalu E, Tamm E, Suvi S, et al. Evaluation of the 124-plex SNP typing microarray for forensic testing. Forensic Sci. Int. Genet. 2009;4:43–48
  11. Magnuson VL, Ally DS, Nylund SJ, Karanjawala ZE, Rayman JB, Knapp JI, et al. Substrate nucleotide-determined non-templated addition of adenine by Taq DNA polymerase: implications for PCR-based genotyping and cloning. Biotechniques. 1996;21:700–709
  12. Børsting C, Rockenbauer E, Morling N. Validation of a single nucleotide polymorphism (SNP) typing assay with 49 SNPs for forensic genetic testing in a laboratory accredited according to the ISO 17025 standard. Forensic Sci. Int. Genet. 2009;4:34–42
  13. AB SNPlex™ chemistry guide pdf, http://docs.appliedbiosystems.com/pebiodocs/04360856.pdf.
  14. Stangegaard M, Tomas C, Hansen AJ, Frank-Hansen R, Børsting C, Morling N. Biomek-3000 and GenPlex SNP genotyping in forensic genetics. JALA. 2008;13(5):297–303
  15. Phillips C, Fang R, Ballard D, Fondevila M, Harrison C, Hyland F, et al. The SNPforConsortium, evaluation of the Genplex SNP typing systems and a 49plex forensic marker panel. Forensic Sci. Int. Genet. 2007;1:180–185
  16. Tomas C, Stangegaard M, Børsting C, Hansen AJ, Morling N. Typing of 48 autosomal SNPs and amelogenin with GenPlex SNP genotyping system in forensic genetics. Forensic Sci. Int. Genet. 2008;3:1–6
  17. Stangegaard M, Frøslev TG, Frank-Hansen R, Lauersen SS, Jørgensen M, Hansen AJ, et al. Automated extraction of DNA and PCR setup using a Tecan Freedom EVO(R) liquid handler. Forensic Sci. Int. Genet., Suppl. Ser. 2009;2:74–76
  18. Tukey J. Explanatory Data Analysis. Addison-Wesley Publishing Co.; 1977;
  19. Gill P. Application of low copy number DNA profiling. Croat. Med. J. 2001;42(3):229–232

PII: S1872-4973(10)00111-0

doi: 10.1016/j.fsigen.2010.06.007

Forensic Science International: Genetics
Volume 5, Issue 5 , Pages 369-375 , November 2011

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