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Editorial Board
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Development of a rapid, 96-well alkaline based differential DNA extraction method for sexual assault evidence
03 February 2011
Abstract: We present a rapid alkaline lysis procedure for the extraction of DNA from sexual assault evidence that generates purified sperm fraction extracts that yield STR typing results similar to th...
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William R. Hudlow,
Martin R. Buoncristiani
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1-16
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A stochastic model of the processes in PCR based amplification of STR DNA in forensic applications
08 February 2011
Abstract: In forensic DNA profiling use is made of the well-known technique of PCR. When the amount of DNA is high, generally unambiguous profiles can be obtained, but for low copy number DNA stochast...
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Jos Weusten,
Jos Herbergs
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17-25
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Cell free DNA as a component of forensic evidence recovered from touched surfaces
03 February 2011
Abstract: In the course of a criminal investigation, DNA is often recovered from items that have been handled by an individual. Whilst there have been studies investigating the propensity of different...
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Ignacio Quinones,
Barbara Daniel
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26-30
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Assessing a novel room temperature DNA storage medium for forensic biological samples
16 February 2011
Abstract: The ability to properly collect, analyze and preserve biological stains is important to preserving the integrity of forensic evidence. Stabilization of intact biological evidence in cells an...
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Steven B. Lee,
Kimberly C. Clabaugh,
Brie Silva,
Kingsley O. Odigie,
Michael D. Coble,
Odile Loreille,
Melissa Scheible,
Ron M. Fourney,
Jesse Stevens,
George R. Carmody,
Thomas J. Parsons,
Arijana Pozder,
Arthur J. Eisenberg,
Bruce Budowle,
Taha Ahmad,
Russell W. Miller,
Cecelia A. Crouse
et al.
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31-40
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The transfer of touch DNA from hands to glass, fabric and wood
17 February 2011
Abstract: The transfer of DNA from hands to objects by holding or touching has been examined in the past. The main purpose of this study was to examine the variation in the amount of DNA transferred f...
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Dyan J. Daly,
Charlotte Murphy,
Sean D. McDermott
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41-46
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Optimization and validation of a fast amplification protocol for AmpFlSTR® Profiler Plus® for rapid forensic human identification
14 March 2011
Abstract: The goal of this work was to optimize and validate a fast amplification protocol for the multiplex amplification of the STR loci included in AmpFlSTR® Profiler Plus® to expedite human DNA id...
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Nancy Laurin,
Chantal Frégeau
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47-57
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Characterising stutter in forensic STR multiplexes
09 March 2011
Abstract: Stutter is an artefact seen when amplifying short tandem repeats and typically occurs at one repeat unit shorter in length than the parent allele. In forensic analysis, stutter complicates t...
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Clare Brookes,
Jo-Anne Bright,
SallyAnn Harbison,
John Buckleton
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58-63
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Complex mixtures: A critical examination of a paper by Homer et al.
23 March 2011
Abstract: DNA evidence in criminal cases may be challenging to interpret if several individuals have contributed to a DNA-mixture. The genetic markers conventionally used for forensic applications may...
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Thore Egeland,
A. Elida Fonneløp,
Paul R. Berg,
Matthew Kent,
Sigbjørn Lien
et al.
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64-69
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RNA/DNA co-analysis from blood stains—Results of a second collaborative EDNAP exercise
04 April 2011
Abstract: A second collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling was organized by the European DNA Profiling Group (EDNAP). Six human blood stains, two ...
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C. Haas,
E. Hanson,
M.J. Anjos,
W. Bär,
R. Banemann,
A. Berti,
E. Borges,
C. Bouakaze,
A. Carracedo,
M. Carvalho,
V. Castella,
A. Choma,
G. De Cock,
M. Dötsch,
P. Hoff-Olsen,
P. Johansen,
F. Kohlmeier,
P.A. Lindenbergh,
B. Ludes,
O. Maroñas,
D. Moore,
M.-L. Morerod,
N. Morling,
H. Niederstätter,
F. Noel,
W. Parson,
G. Patel,
C. Popielarz,
E. Salata,
P.M. Schneider,
T. Sijen,
B. Sviežena,
M. Turanská,
L. Zatkalíková,
J. Ballantyne
et al.
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70-80
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Divergent genetic strata in five Bahamian islands
11 April 2011
Abstract: Based on historical records, the genetic landscape of the Bahamian archipelago is presumed to be complex and to exhibit island-specific characteristics, yet the genetic composition of the is...
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Tanya M. Simms,
Dianne A. Barrett,
Quinn McCartney,
Rene J. Herrera
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81-90
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Development of a one-tube extraction and amplification method for DNA analysis of sperm and epithelial cells recovered from forensic samples by laser microdissection
16 March 2011
Abstract: Laser microdissection can be used in forensic casework to isolate specific cell types from mixtures of biological samples. Extraction of DNA from selected cells is still required prior to ST...
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Melanie Meredith,
Jo-Anne Bright,
Sarah Cockerton,
Sue Vintiner
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91-96
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Statistical model for degraded DNA samples and adjusted probabilities for allelic drop-out
04 April 2011
Abstract: DNA samples found at a scene of crime or obtained from the debris of a mass disaster accident are often subject to degradation. When using the STR DNA technology, the DNA profile is observed...
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Torben Tvedebrink,
Poul Svante Eriksen,
Helle Smidt Mogensen,
Niels Morling
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97-101
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Extended PCR conditions to reduce drop-out frequencies in low template STR typing including unequal mixtures
01 April 2011
Abstract: Forensic laboratories employ various approaches to obtain short tandem repeat (STR) profiles from minimal traces (<100pg DNA input). Most approaches aim to sensitize DNA profiling by incr...
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Natalie E.C. Weiler,
Anuska S. Matai,
Titia Sijen
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102-107
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Discordance at D3S1358 locus involving SGM Plus™ and the European new generation multiplex kits
07 April 2011
Abstract: During the course of routine database sample analysis in the Israel Police DNA database, an off-ladder D3S1358 allele, calculated to be >22.1, extending into the adjacent vWA locus was ob...
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Aliza Raziel,
Carla Oz,
Aviva Dell’Ariccia Carmon,
Rafi Ilsar,
Ashira Zamir
et al.
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108-112
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Belgian canine population and purebred study for forensics by improved mitochondrial DNA sequencing
14 April 2011
Abstract: In canine population studies for forensics, the mitochondrial DNA is profiled by sequencing the two hyper variable regions, HV1 and HV2 of the control region. In a first effort to create a B...
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Stijn Desmyter,
Leonie Gijsbers
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113-120
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An unusual case of identification by DNA analysis of siblings
28 March 2011
Abstract: A badly decomposed body required identification by means of DNA analysis. A brother and sister of the deceased were available as reference subjects. Although investigation of Y-chromosomal m...
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Franz Neuhuber,
Max P. Baur,
Jan Cemper-Kiesslich,
Bettina Dunkelmann,
Fabio Monticelli
et al.
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121-123
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| Short Communications |
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Developmental validation of the PowerPlex® ESX 16 and PowerPlex® ESX 17 Systems
06 April 2011
Abstract: We describe the developmental validation study performed on the PowerPlex® ESX 16 (European Standard Extended 16) and the PowerPlex® ESX 17 Systems, part of a suite of four new DNA profiling...
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Valerie C. Tucker,
Andrew J. Hopwood,
Cynthia J. Sprecher,
Robert S. McLaren,
Dawn R. Rabbach,
Martin G. Ensenberger,
Jonelle M. Thompson,
Douglas R. Storts
et al.
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124-131
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Allelic frequencies and statistical data obtained from 48 AIM INDEL loci in an admixed population from the Brazilian Amazon
03 May 2011
Abstract: Allelic frequencies of 48 informative insert-delete (INDEL) loci were obtained from a sample set of 130 unrelated individuals living in Macapá, a city located in the northern Amazon region, ...
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Pablo Abdon da Costa Francez,
Elzemar Martins Ribeiro-Rodrigues,
Sidney Emanuel Batista dos Santos
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132-135
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Distribution of Y chromosomal STRs loci in Mayan and Mestizo populations from Guatemala
12 May 2011
Abstract: In this study, a sample of 225 Guatemalan males, comprising 115 Mestizo-Guatemalan and 110 Mayan-Guatemalan, was typed for 17 Y-short tandem repeats (STRs) loci (DYS19, DYS389I, DYS389II, DY...
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Luis J. Martínez-González,
María Saiz,
María J. Álvarez-Cubero,
Antonio Gómez-Martín,
Juan C. Álvarez,
Cristina Martínez-Labarga,
José A. Lorente
et al.
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136-142
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Current Next Generation Sequencing technology may not meet forensic standards
12 May 2011
Abstract: In a Nature paper of 2010, the concern was raised that intra-individual mtDNA variation may be more pronounced than previously believed, in that heteroplasmies are common and vary markedly f...
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Hans-Jürgen Bandelt,
Antonio Salas
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143-145
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| Online extra |
| Letters to the Editor |
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The role of probabilities in graphical models
09 November 2010
We have read with great interest the paper “A universal strategy to interpret DNA profiles that does not require a definition of low-copy-number” by Peter Gill and John Buckleton (Forensic Science Int...
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A. Biedermann,
F. Taroni
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e1-e2
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Allele frequencies of fifteen STR loci in U.S. immigrants from Haiti compared with African Americans and Afro-Caribbeans
06 December 2010
About 20,000 Haitians emigrate to the United States each year. Many are beneficiaries of family-based petitions from naturalized U.S. citizens . Since Haitian birth and marriage records are often none...
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Amie Branham,
Robert Wenk,
Francis Chiafari
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e3-e4
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Genetic analysis of 17 Y-chromosomal STRs haplotypes of three ethnic groups residing in West Bengal, India
13 December 2010
We have analyzed 17 Y-chromosomal STR loci (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS393, DYS391, DYS439, DYS635, DYS392, Y-GATA-H4, DYS437, DYS438, DYS448 and DYS385a/b) in 125 unrelated ...
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Soma Roy,
Sabahat Noor,
I. Haque
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e5-e7
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The Israel Police DNA database: Recognizing the capability of databases in providing investigative leads
22 December 2010
The Israel Police DNA database has been operative since February 2007. During this period of operation a number of criminal cases have been encountered and resolved not by direct cold hits, but by fol...
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Carla Oz,
Merav Amiel,
Ashira Zamir
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e8-e10
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Allele frequencies of nine non-CODIS STR loci in Chinese Uyghur ethnic minority group
27 January 2011
We investigated the genetic polymorphisms of nine non-combined DNA index system (CODIS) short tandem repeat (STR) loci for 252 healthy unrelated autochthonous individuals of Chinese Uyghur ethnic mino...
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Siyue Li,
Yi Zhou,
Zhao Cheng,
Jun Wang,
Xingbo Song,
Yuanxin Ye,
BinWu Ying,
An Zhenmei
et al.
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e11-e12
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Population data for 10 X-chromosome STRs from north-east of Spain
04 February 2011
Autosomic and chromosome Y STRs markers have been widely applied in forensic identity and paternity test, however, the application of X chromosome STR markers plays a minor role in forensic casework. ...
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Belén García,
Manuel Crespillo,
Miguel Paredes,
Juan L. Valverde
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e13-e15
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Bracketing off population does not advance ethical reflection on EVCs: A reply to Kayser and Schneider
01 February 2011
In a recent contribution to this journal, Kayser and Schneider reviewed the relevance of external visible characteristics (EVCs) for criminal investigation . Their aim was to broaden the debate about ...
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Amade M’charek,
Victor Toom,
Barbara Prainsack
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e16-e17
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Reply to “Bracketing off population does not advance ethical reflection on EVCs: A reply to Kayser and Schneider” by A. M’charek, V. Toom, and B. Prainsack
14 February 2011
We appreciate the letter by M’charek et al. regarding our publication on DNA-based predictions of human externally visible characteristics (EVCs) in forensics . It indeed was one of the intentions of...
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Manfred Kayser,
Peter M. Schneider
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e18-e19
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Genetic variation of 15 autosomal STR loci in various populations from southern Africa
31 January 2011
We determined the allele frequencies for the 15 autosomal STR loci included in the AmpFlSTR® Identifiler™ kit in 324 unrelated individuals from southern African, representing San, Khoe, Coloured and B...
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Carina M. Schlebusch,
Himla Soodyall,
Mattias Jakobsson
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e20-e21
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Population genetics polymorphisms on 17 autosomal STRs from Chinese Bai ethnic minority group
31 January 2011
We studied the genetic polymorphisms and evaluated the forensic application values of 17 autosomal short tandem repeat (STR) loci. We have co-amplified and analyzed the 13 loci STRs included in the Co...
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Shuan-liang Fan,
Chun-mei Shen,
Fang Wang,
Jun Ma,
Hong-dan Wang,
Guang Yang,
Hai-xia Qin,
Tong Xie,
Shao-bo Li,
Jiang-wei Yan,
Yao Liu
et al.
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e22-e23
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X-chromosomal haplotype frequencies of four linkage groups using the Investigator Argus X-12 Kit
03 February 2011
The Investigator Argus X-12 Kit allows simultaneous amplification of 12 X-chromosomal STR loci which are highly informative for kinship and paternity testing, population genetics and anthropological s...
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Jeanett Edelmann,
Sabine Lutz-Bonengel,
Jana Naue,
Sandra Hering
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e24-e34
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Forensic genetic data of 6 Y-STR loci: An expanded Korean population database
04 February 2011
Applications of Y-chromosomal DNA analysis in forensic casework benefit from large population databases for estimating the probability of identity by chance. Thus, it is important that Y-STR databases...
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Soon Hee Kim,
Seung Beom Hong,
Kyoung Don Kwak,
Eun Jung Lee,
Jong Jin Kim,
Ki Cheol Kim,
Han Jun Jin,
Myun Soo Han,
Wook Kim,
Won Kim
et al.
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e35-e36
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Allele frequencies of the new European Standard Set (ESS) loci in a population of Southern Italy (Calabria)
14 March 2011
Allele frequencies of 5 STRs loci (D10S1248, D2S441, D1S1656, D12S391, and D22S1045) included in the new European Standard Set (ESS) were calculated in Calabria (South of Italy) population using the n...
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Anna Barbaro,
Patrizia Cormaci,
Stefano Votano,
Alessandro Agostino
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e37-e38
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Genetic characterization of Western Iberia using Mentype® Argus X-8 kit
18 April 2011
In order to build a X-STR database in individuals from Western Iberia, 327 samples (83 females and 244 males) were genotyped by the use of Mentype® Argus X-8 kit. In particular, a total of 410 X chrom...
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Miguel Gelabert-Besada,
Cíntia Alves,
Suelen Ferreira,
Manuel García-Magariños,
Leonor Gusmão,
Paula Sánchez-Diz
et al.
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e39-e41
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Genetic data for D1S1677, D2S441, D4S2364, D10S1248, D14S1434 and D22S1045 miniSTR loci from the state of Rio Grande do Sul, Southern Brazil
14 April 2011
We determined the allelic frequencies for six miniSTR loci D10S1248, D14S1434, D22S1045 (miniplex NC01) and D1S1677, D2S441, D4S2364 (miniplex NC02) in a sample of 439 unrelated individuals from the R...
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Paulo Eduardo Raimann,
Ana Paula Aidar de Oliveira,
Rodrigo Rodenbusch,
Juliane Bentes Picanço,
Trícia Cristine Kommers Albuquerque,
Clarice Sampaio Alho
et al.
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e42-e43
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Genetic data of 15 STR loci in five populations from Afghanistan
13 April 2011
The variability of 15 hyper-variable autosomal STR loci included in the AmpFlSTR Identifiler™ PCR amplification kit (Applied Biosystems) was analyzed in Afghan individuals from five populations locate...
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Julie Di Cristofaro,
Stéphane Buhler,
Shah Aga Temori,
Jacques Chiaroni
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e44-e45
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A genetic study of 12 X-STR loci in the Hungarian population
06 April 2011
Males carry one X-chromosome, thus they transmit their X-chromosome to daughters as haplotypes. Females carry two X-chromosomes which are liable to recombination during meiosis, thus this must be take...
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Gergely Horváth,
Andrea Zalán,
Zoltán Kis,
Horolma Pamjav
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e46-e47
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Genetic diversity of 12 X-chromosomal short tandem repeats in the Moroccan population
04 April 2011
Morocco is a country located in northwest Africa. It has a long coastline on the Atlantic Ocean that reaches past the Strait of Gibraltar into the Mediterranean Sea. Morocco has been the home of the B...
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Kaoutar Bentayebi,
Antònia Picornell,
Mehdi Bouabdeallah,
José Aurelio Castro,
Rachid Aboukhalid,
Driss Squalli,
Maria Misericòrdia,
Saaïd Amzazi
et al.
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e48-e49
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Allele frequencies and concordance study of 16 STR loci – including the new European Standard Set (ESS) loci – in an Austrian population sample
02 May 2011
National DNA databases have been established as an important crime prevention tool in many European countries. The increasing exchange of DNA-profiles, which is facilitated by the treaty of Prüm and t...
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P. Hatzer-Grubwieser,
B. Berger,
D. Niederwieser,
M. Steinlechner
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e50-e51
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Expanding the CODIS core loci in the United States
05 May 2011
After over a decade of operation, the National DNA Index System (NDIS) continues to grow in importance and size . While the STR DNA technology has remained relatively consistent, other key aspects of ...
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Douglas R. Hares
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e52-e54
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Allele frequencies of the five new generation forensic STR (D1S1656, D2S441, D10S1248, D12S391 and D22S1045) in the population from Rio Grande do Sul, Southern Brazil
04 July 2011
Allele frequencies of the five recently introduced European Standard Set (ESS) STRs markers (D1S1656, D2S441, D10S1248, D12S391 and D22S1045) were obtained from a sample of 353 individuals undergoin...
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Rodrigo Rodenbusch,
Simone Schumacher,
Ana Carolina Mardini,
Fernanda Goulart Lanes Chula,
Candice Tosi Michelon,
Candice Tosi Michelon,
André Zoratto Gastaldo,
Lila Particheli Maciel,
Gabriela C.R. Hirschfeld-Campolongo,
Paulo Eduardo Raimann,
Sabrina Esteves de Matos Almeida,
Cláudia Maria Dornelles da Silva
et al.
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e55-e57
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Allele frequencies of 15 STRs in the Calchaqui Valleys population (North-Western Argentina)
06 June 2011
Abstract: Allele frequencies for 15 short tandem repeat (STR) loci were obtained from a sample of 110 individuals from the Calchaqui Valleys population (North-Western Argentina). The combined power of...
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A. Muñoz,
M.V. Albeza,
N. Acreche,
J.A. Castro,
M.M. Ramon,
A. Picornell
et al.
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e58-e60
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A paternity case with mutations at three CODIS core STR loci
03 June 2011
Short tandem repeat (STR) is the most widely used genetic marker in the paternity identification. Mutations of STR loci have been observed and reported during the paternity testing . However, most of ...
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Hong-yu Sun,
Hai-xia Li,
Xiang-pei Zeng,
Zheng Ren,
Wen-jing Chen
et al.
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e61-e62
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