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Research paper| Volume 23, P240-247, July 2016

DNA transfer during laundering may yield complete genetic profiles

      Highlights

      • DNA from semen or vaginal secretions can transfer among items during laundering.
      • Both sources may transfer enough DNA to yield complete genetic profiles.
      • Even with no sexual event DNA from both parents was found on their children’s panties.
      • A framework for the handling of intrafamilial sexual abuse cases is proposed.

      Abstract

      In a number of child sexual abuse cases, the alleged perpetrator is a member of the nuclear family. In those cases, there is a possibility that the suspect’s DNA was innocently deposited onto the child’s clothing without acts of sexual assault ever occurring, for example via secondary transfer within the washing machine. To assess the quantity and quality of DNA that may be transferred among clothing during laundering, we conducted three series of experiments. First, we evaluated the level of spermatozoa that may be transferred by washing pristine pairs of underwear with bed sheets containing a varying number of ejaculates. Secondly, we explored whether current genetic methods may also detect the transfer of DNA from vaginal secretions during a machine wash. Finally, we analyzed the background levels of DNA on children’s underwear collected from control families where sexual abuse never occurred. For both spermatozoa and vaginal secretions, we revealed that sufficient amounts of DNA may transfer onto laundered clothing to yield complete genetic profiles. Furthermore, DNA from relatives living within the same household was found in most cuttings taken from control children’s underwear. Based on these findings, we present a framework for the handling and interpretation of intrafamilial sexual abuse cases. These suggestions should help determine whether DNA was deposited directly onto a fabric or merely transferred during a wash.

      Graphical abstract

      Keywords

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      References

        • Christian C.W.
        • Lavelle J.M.
        • De Jong A.R.
        • Loiselle J.
        • Brenner L.
        • Joffe M.
        Forensic evidence findings in prepubertal victims of sexual assault.
        Pediatrics. 2000; 106: 100-104https://doi.org/10.1542/peds.106.1.100
        • Young K.L.
        • Jones J.G.
        • Worthington T.
        • Simpson P.
        • Casey P.H.
        Forensic laboratory evidence in sexually abused children and adolescents.
        Arch. Pediatr. Adolesc. Med. 2006; 160: 585-588https://doi.org/10.1001/archpedi.160.6.585
        • Willott G.M.
        • Allard J.E.
        Spermatozoa – their persistence after sexual intercourse.
        Forensic Sci. Int. 1982; 19: 135-154https://doi.org/10.1016/0379-0738(82)90040-8
        • Girardet R.
        • Bolton K.
        • Lahoti S.
        • Mowbray H.
        • Giardino A.
        • Isaac R.
        • Arnold W.
        • Mead B.
        • Paes N.
        Collection of forensic evidence from pediatric victims of sexual assault.
        Pediatrics. 2011; 128: 233-238https://doi.org/10.1542/peds.2010-3037
        • Dziak R.
        • Parker L.
        • Collins V.
        • Johnston S.
        Providing evidence based opinions on time since intercourse (TSI) based on body fluid testing results of internal samples.
        Can. Soc. Forensic Sci. J. 2011; 44: 59-69https://doi.org/10.1080/00085030.2011.10768142
        • Magalhães T.
        • Taveira F.
        • Jardim P.
        • Santos L.
        • Matos E.
        • Santos A.
        Sexual abuse of children. A comparative study of intra and extra-familial cases.
        J. Forensic Leg. Med. 2009; 16: 455-459https://doi.org/10.1016/j.jflm.2009.05.007
        • Palusci V.J.
        • Cox E.O.
        • Shatz E.M.
        • Schultze J.M.
        Urgent medical assessment after child sexual abuse.
        Child Abuse Negl. 2006; 30: 367-380https://doi.org/10.1016/j.chiabu.2005.11.002
        • Thackeray J.D.
        • Hornor G.
        • Benzinger E.A.
        • Scribano P.V.
        Forensic evidence collection and DNA identification in acute child sexual assault.
        Pediatrics. 2011; 128: 227-232https://doi.org/10.1542/peds.2010-3498
        • Gingras F.
        • Paquet C.
        • Bazinet M.
        • Granger D.
        • Marcoux-Legault K.
        • Fiorillo M.
        • Séguin D.
        • Baltzer F.
        • Chamberland C.
        • Jolicoeur C.
        Biological and DNA evidence in 1000 sexual assault cases.
        Forensic Sci. Int. 2009; 2: 138-140https://doi.org/10.1016/j.fsigss.2009.09.006
        • Kafarowski E.
        • Lyon A.M.
        • Sloan M.M.
        The retention and transfer of spermatozoa in clothing by machine washing.
        Can. Soc. Forensic Sci. J. 1996; 29: 7-11https://doi.org/10.1080/00085030.1996.10757042
        • Crowe G.
        • Moss D.
        • Elliot D.
        The effect of laundering on the detection of acid phosphatase and spermatozoa on cotton T-shirts.
        Can. Soc. Forensic Sci. J. 2000; 33: 1-5https://doi.org/10.1080/00085030.2000.10757498
        • Jobin R.M.
        • Gouffe M.D.
        The persistence of seminal constituents on panties after laundering. Significance to investigations of sexual assault.
        Can. Soc. Forensic Sci. J. 2003; 36: 1-10https://doi.org/10.1080/00085030.2003.10757551
        • Farmen R.K.
        • Cortez P.
        • Frøyland E.S.
        Spermatozoa recovered on laundered clothing.
        Forensic Sci. Int. 2008; 1: 418-420https://doi.org/10.1016/j.fsigss.2007.10.218
        • Spector J.
        • Von Gemmingen D.
        The effect of washing on the detection of blood and seminal stains.
        Can. Soc. Forensic Sci. J. 1971; 4: 3-9https://doi.org/10.1080/00085030.1971.10757279
        • Brayley-Morris H.
        • Sorrell A.
        • Revoir A.P.
        • Meakin G.E.
        • Court D.S.
        • Morgan R.M.
        Persistence of DNA from laundered semen stains: implications for child sex trafficking cases.
        Forensic Sci. Int. 2015; 19: 165-171https://doi.org/10.1016/j.fsigen.2015.07.016
      1. S. Noël, A. Rogic, K. Lagacé, D. Granger, S. Bourgoin, M. Loyer, C. Jolicoeur, D. Séguin, Is it fresh? Comparative serological and genetic properties of washed and unwashed semen stains (2016) In prep. .

        • Joshi U.N.
        • Subhedar S.K.
        • Saraf D.K.
        Effect of water immersion on seminal stains on cotton cloth.
        Forensic Sci. Int. 1981; 17: 9-11
        • Kamphausen T.
        • Fandel S.B.
        • Gutmann J.S.
        • Bajanowski T.
        • Poetsch M.
        Everything clean? Transfer of DNA traces between textiles in the washtub.
        Int. J. Leg. Med. 2015; 129: 709-714https://doi.org/10.1007/s00414-015-1203-5
        • Kelly K.G.
        Tests on vaginal discharge.
        in: Walker H.K. Hall W.D. Hurst J.W. Clinical Methods: The History, Physical, and Laboratory Examinations. Butterworths, Boston2011
        • Dennerstein L.
        • Dudley E.C.
        • Hopper J.L.
        • Guthrie J.R.
        • Burger H.G.
        A prospective population-based study of menopausal symptoms.
        Obstetrics Gynecol. 2000; 96: 351-358https://doi.org/10.1016/S0029-7844(00)00930-3
        • Andrews C.
        • Coquoz R.
        PCR DNA typing of washed stains.
        Advances in Forensic Haemogenetics. Springer, Berlin, Heidelberg1994: 343-345
        • Goray M.
        • Eken E.
        • Mitchell R.J.
        • van Oorschot R.A.H.
        Secondary DNA transfer of biological substances under varying test conditions.
        Forensic Sci. Int. Genet. 2010; 4: 62-67https://doi.org/10.1016/j.fsigen.2009.05.001
        • Boyce J.
        • Cotter A.
        • Perreault S.
        Police-reported Crime Statistics in Canada.
        Canadian Centre for Justice Statistics, 2013
        • Finkelhor D.
        • Jones L.
        • Shattuck A.
        • Saito K.
        Updated Trends in Child Maltreatment, 2012.
        Crimes against Children Research Center, 2013