Abstract
Multiplex human short tandem repeat analysis demands reliable DNA quantification to
consistently produce interpretable genotypes. The Plexor® HY System is a multiplex quantitative PCR assay to quantify total human and male
DNA. We performed developmental validation of the Plexor® HY System to demonstrate the performance capabilities and limitations of the assay
for forensic applications. Validation studies examined: (a) human specificity, (b)
sensitivity, (c) quantification of degraded DNA, (d) impact of inhibitors, (e) male/female
mixture and Y-assay male specificity, (f) reproducibility and concordance and (g)
population studies.
Keywords
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Article info
Publication history
Published online: September 11, 2008
Accepted:
July 10,
2008
Received in revised form:
July 9,
2008
Received:
March 17,
2008
Identification
Copyright
© 2008 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
