Abstract
STR investigations of telogen hair are invariably difficult due to the small amounts
of nuclear DNA and its degradation products. However, in recent years there has been
a considerable improvement. This study examined the suitability of a new STR kit with
shortened amplicons for the investigation of hair in routine casework. This kit allows
the simultaneous amplification of the eight STR-loci D3S1358, VWA, FGA, TH01, SE33,
D8S1179, D18S51, and D21S11, and the sex-determining amelogenin system. It was tested
against the genRES® MPX-SP1 and genRES® MPX-SP2 kits. The sensitivity of the new genRES® MPX-2SP kit was demonstrated to be inferior to that of the genRES® MPX-SP1, but almost equal to that of the genRES® MPX-SP2 kit.
Keywords
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References
- Extraction, PCR amplification and sequencing of mitochondrial DNA from human hair shafts.Biotechniques. 1995; 18: 662-669
- Mitochondrial DNA typing from human axillary, pubic and head hair shafts—success rates and sequence comparisons.Int. J. Legal Med. 1999; 112: 287-290
- Forensic mitochondrial DNA analysis of 691 casework hairs.J. Forensic Sci. 2005; 50: 73-80
- STR typing of human hairs—a new approach.Int. J. Legal Med. 2001; 114: 269-273
- The development of reduced size STR amplicons as tools for analysis of degraded DNA.J. Forensic Sci. 2003; 48: 1054-1064
- Short tandem repeat (STR) genotyping of keratinised hair. Part 1. Review of current status and knowledge gaps.Forensic Sci. Int. 2005; 153: 237-246
- Short tandem repeat (STR) genotyping of keratinised hair. Part 2. An optimised genomic DNA extraction procedure reveals donor dependence of STR profiles.Forensic Sci. Int. 2005; 153: 247-259
- Validation and casework testing of the BioPlex-11 for STR typing of telogen hair roots.Forensic Sci. Int. 2006; 161 (Epub 2005): 52-59
- Short amplicon STR multiplex for stain typing.Int. J. Legal Med. 2006; 120: 160-164
- Comparative investigation of hair with the genRES® MPX-SP1, genRES® MPX-SP2 and genRES® MPX-2 kits.Am. J. Forensic Med. Pathol. 2007; 3: 41-44
Article info
Publication history
Published online: August 11, 2008
Accepted:
July 3,
2008
Received in revised form:
June 17,
2008
Received:
July 31,
2007
Identification
Copyright
© 2008 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.