Abstract
17 Y-STRs (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391,
DYS439, DYS635 or Y-GATA C4, DYS392, Y-GATA H4, DYS437, DYS438 and DYS448) have been
analyzed in 320 male individuals from Sarawak, an eastern state of Malaysia on the
Borneo island using the AmpFlSTR® Y-filer™ (Applied Biosystems, Foster City, CA). These individuals were from three
indigenous ethnic groups in Sarawak comprising of 103 Ibans, 113 Bidayuhs and 104
Melanaus. The observed 17-loci haplotypes and the individual allele frequencies for
each locus were estimated, whilst the locus diversity, haplotype diversity and discrimination
capacity were calculated in the three groups. Analysis of molecular variance (AMOVA)
indicated that 87.6% of the haplotypic variation was found within population and 12.4%
between populations (fixation index FST = 0.124, p = 0.000). This study has revealed that the indigenous populations in Sarawak are distinctly
different to each other, and to the three major ethnic groups in Malaysia (Malays,
Chinese and Indians), with the Melanaus having a strikingly high degree of shared
haplotypes within. There are rare unusual variants and microvariants that were not
present in Malaysian Malay, Chinese or Indian groups. In addition, occurrences of
DYS385 duplications which were only noticeably present in Chinese group previously
was also observed in the Iban group whilst null alleles were detected at several Y-loci
(namely DYS19, DYS392, DYS389II and DYS448) in the Iban and Melanau groups.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Forensic Science International: GeneticsAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- DNA Commission of the International Society of Forensic Genetics: recommendations on forensic analysis using Y-chromosome STRs.Forensic Sci. Int. 2001; 124: 5-10
- Molecular Evolutionary Genetics.Columbia University Press, New York, USA1987
- Arlequin ver. 3.0: an integrated software package for population genetics data analysis.Evolutionary Bioinformatics Online. 2005; 1: 47-50
- Haplotype diversity of 16 Y-chromosomal STRs in three main ethnic populations (Malays, Chinese and Indians) in Malaysia.Forensic Sci. Int. 2007; 167: 70-76
- DNA Commission of the International Society of Forensic Genetics (ISFG): an update of the recommendations on the use of Y-STRs in forensic analysis.Forensic Sci. Int. 2006; 157: 187-197
- Structural variation on the short arm of the human Y chromosome: recurrent multigene deletions encompassing Amelogenin Y.Hum. Mol. Genet. 2007; 16: 307-316
- Inadvertent diagnosis of male infertility through genealogical DNA testing.J. Med. Genet. 2005; 42: 366-368
- Chromosomal duplications along the Y-chromosome and their potential impact on Y-STR interpretation.J. Forensic Sci. 2005; 50: 853-859
- (http://en.wikipedia.org/wiki/Colony_of_Sarawak)
- Publication of population data of human polymorphisms.Forensic Sci. Int. 2000; 110: 3-5
Article info
Publication history
Published online: September 11, 2008
Accepted:
July 23,
2008
Received in revised form:
June 2,
2008
Received:
February 17,
2008
Identification
Copyright
© 2008 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
