Abstract
About 50 Chrysomyinae specimens belonging to three forensic relevant species (Chrysomia albiceps, Phormia regina and Protophormia terraenovae) were collected from different geographical locations in Belgium over the last 5 yr. A 304-bp fragment of their mitochondrial COI gene is sequenced. The monophyletic
branches of the phylogenetic tree reveal that this marker is suitable for discrimination
between these species. The intra versus interspecific variability marks clear threshold
levels for DNA barcoding. Nineteen Chrysomyinae specimens, collected from four locations
in France, show mitotypes that are identical or at least very similar to the Belgian
mitotypes. Considering additional specimens from outside of Europe reveals no intraspecific
geographical variation within C. albiceps and P. terraenovae, whereas P. regina is subbranched in a Belgian–French and a USA–Chinese population.
Keywords
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Article info
Publication history
Published online: January 16, 2009
Accepted:
November 14,
2008
Received in revised form:
October 27,
2008
Received:
August 25,
2008
Identification
Copyright
© 2008 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
