Abstract
Analysis of Y chromosome STR markers has proven to be useful in forensic cases where
the samples contain a mixture of DNA from several individuals. STR markers are commonly
genotyped based on length separation of PCR products. In this study we evaluated if
Pyrosequencing can be used as an alternative method for determining Y-STR variants.
In total 70 unrelated Swedish males were typed for the Y chromosomal markers (DYS19,
DYS389 I-II, DYS390, DYS391, DYS392, DYS393 and DYS438) using Pyrosequencing. Using
the 8 markers, 57 unique haplotypes were observed with a discrimination capacity of
0.81. At four loci, the Pyrosequencing analysis revealed sequence variants. The sequence
variants were found in the DYS389 II, DYS390, DYS391, and DYS393 loci in frequencies
between 1.43% and 14.3%. Pyrosequencing has here been shown to be a useful tool for
typing Y chromosomal STRs and the method can provide a complement to conventional
forensic Y STR analyses. Moreover, the Pyrosequencing method can be used to rapidly
evaluate novel markers.
Keywords
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Article info
Publication history
Published online: January 07, 2009
Accepted:
November 19,
2008
Received in revised form:
November 19,
2008
Received:
August 26,
2008
Identification
Copyright
© 2008 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
