Abstract
DNA isolation is the initial step of most genetic studies, and ideally it should use
a reliable and non-invasive method. Buccal samples are adequate for such purposes,
being painless, easy to collect and a very reliable DNA source. FTA® Elute Cards are relatively new on the market and are designed for rapid blood DNA
extraction, in which DNA is solubilized in water, instead of attached to the paper
matrix. In this study, we sought to test if FTA® Elute Cards are suitable for buccal DNA extraction. Furthermore, several time and
temperature conditions were analyzed in order to determine the best concentration/time
ratio. Twenty-five different conditions were tested in quadruplicate and extracted
DNA was quantified by real-time PCR.
Keywords
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Article info
Publication history
Published online: January 16, 2009
Accepted:
November 24,
2008
Received in revised form:
October 27,
2008
Received:
August 12,
2008
Identification
Copyright
© 2008 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
