DNA isolation is the initial step of most genetic studies, and ideally it should use a reliable and non-invasive method. Buccal samples are adequate for such purposes, being painless, easy to collect and a very reliable DNA source. FTA® Elute Cards are relatively new on the market and are designed for rapid blood DNA extraction, in which DNA is solubilized in water, instead of attached to the paper matrix. In this study, we sought to test if FTA® Elute Cards are suitable for buccal DNA extraction. Furthermore, several time and temperature conditions were analyzed in order to determine the best concentration/time ratio. Twenty-five different conditions were tested in quadruplicate and extracted DNA was quantified by real-time PCR.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Forensic Science International: Genetics
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- Collection of blood, saliva, and buccal cell samples in a pilot study on the Danish nurse cohort: comparison of the response rate and quality of genomic DNA.Cancer Epidemiol. Biomarkers Prev. 2007; 16: 2072-2076
- Saliva as a viable alternative source of human genomic DNA in genetic epidemiology.Clin. Chim. Acta. 2006; 367: 81-85
- Buccal DNA collection: comparison of buccal swabs with FTA cards.Cancer Epidemiol. Biomarkers Prev. 2006; 15: 816-819
- Collection of buccal cell DNA using treated cards.Cancer Epidemiol. Biomarkers Prev. 2000; 9: 501-506
- Application of FTA technology to extraction of sperm DNA from mixed body fluids containing semen.Legal Med. 2006; 8: 43-47
- Automated forensic DNA purification optimized for FTA card punches and identifiler STR-based PCR analysis.Clin. Lab. Med. 2007; 27: 183-191
- Optimized pH method for DNA elution from buccal cells collected in Whatman FTA cards.Genet. Test. 2006; 10: 126-130
- Improved technique that allows the performance of large-scale SNP genotyping on DNA immobilized by FTA® technology.Infect. Genet. Evol. 2007; 7: 128-132
- Quantification of mixed chimerism by real time PCR on whole blood-impregnated FTA cards.Leuk. Res. 2007; 31: 1175-1183
- Buccal swabs and treated cards: methodological considerations for molecular epidemiologic studies examining pediatric populations.Am. J. Epidemiol. SW. 2008; : 1-8
- Collecting, archiving and processing DNA from wildlife samples using FTA® databasing paper.BMC Ecol. 2004; 4: 1-11
- Comparison of methods for DNA extraction from paraffin-embedded tissues and buccal cells.Cancer Detect. Prev. 2003; 27: 397-404
- Collection of genomic DNA by buccal swabs for polymerase chain reaction-based biomarker assays.Environ. Health Perspect. 1999; 107: 517-520
- Evaluation of saliva as a source of human DNA for population and association studies.Anal. Biochem. 2006; 353: 272-277
Published online: January 16, 2009
Accepted: November 24, 2008
Received in revised form: October 27, 2008
Received: August 12, 2008
© 2008 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.