Abstract
Use of Amelogenin locus typing as a gender marker incorporated in STR multiplexes
is common practice in forensic genetics analysis. Among 5534 Polish male individuals
tested using the SGMPlus kit, one was found to lack the amelogenin X-specific homologue
(0.02%). The same result was obtained with other commercial kits which also amplify
the amelogenin locus, namely ProfilerPlus and PowerPlex16. When alternative amelogenin
primers external to but encompassing the initial amplicon were applied, an X homologue
product was seen. Sequencing of the X homologue amelogenin allele revealed C to G
mutation located at the most 3′ base of the commonly used amelogenin reverse primer.
To our knowledge, this mutation and failure to amplify the X homologue of the amelogenin
gene has not been reported for the European population.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Forensic Science International: GeneticsAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Reliability of DNA-based sex tests.Nat. Genet. 1998; 18: 103
- Rare failures in the Amelogenin sex test.Int. J. Legal Med. 2002; 116: 117-120
- Is the Amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis?.Int. J. Legal Med. 2002; 116: 121-123
- A large interstitial deletion encompassing the Amelogenin gene on the short arm of the Y chromosome.Hum. Genet. 2005; 116: 395-401
- Erroneous gender identification by the Amelogenin sex test.J. Forensic Sci. 2004; 49: 258-259
- Anomalous amplification of the amelogenin locus typed by AmpFLSTR ProfilerPlus Amplification Kit.Forensic Sci. Commun. 2000; 2
- A rare mutation in the primer binding region of the Amelogenin gene can interfere with gender identification.J. Mol. Diagn. 2004; 6: 401-405
- The Amelogenin locus displays a high frequency of X homologue failures in Sao Tome Island (West Africa).Int. Congr. Ser. 2006; 1288: 271-273
- Sex identification by polymerase chain reaction using X–Y homologous primer.Am. J. Med. Genet. 1991; 39: 472-473
- A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X–Y homologous gene amelogenin.Biotechniques. 1993; 15: 636-641
- Discontinuous polyacrylamide gel electrophoresis of DNA fragments.in: Mathew C. Methods in Molecular Biology, Vol 9. Protocols in Human Molecular Genetics. The Humana Press Inc., Clifton, NJ1991: 123-132
Article info
Publication history
Published online: February 24, 2009
Accepted:
January 19,
2009
Received in revised form:
January 9,
2009
Received:
November 17,
2008
Identification
Copyright
© 2009 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
