Abstract
In this study we examine the limitations of currently used sequence-based approaches
to blowfly (Calliphoridae) identification and evaluate the utility of an immunological
approach to discriminate between blowfly species of forensic importance. By investigating
antigenic similarity and dissimilarity between the first instar larval stages of four
forensically important blowfly species, we have been able to identify immunoreactive
proteins of potential use in the development of species-specific immuno-diagnostic
tests. Here we outline our protein-based approach to species determination, and describe
how it may be adapted to develop rapid diagnostic assays for the ‘on-site’ identification
of blowfly species.
Keywords
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References
- A manual of forensic entomology.The British Museum (Natural History). London and Cornell University Press, 1986 (p. 204)
- DNA typing for identification of some species of Calliphoridae—an interest in forensic entomology.Forensic Science International. 1999; 102: 111-119
- Molecular identification of forensically important blow fly species (Diptera: Calliphoridae) in Taiwan.Journal of Medical Entomology. 2004; 41: 47-57
- Mitochondrial DNA cytochrome oxidase I gene: potential for distinction between immature stages of some forensically important fly species (Diptera) in western Australia.Forensic Science International. 2003; 131: 134-139
- The forensic application of allozyme electrophoresis to the identification of blowfly larvae (Diptera: Calliphoridae) in southern Australia.Journal of Forensic Sciences. 2001; 46: 681-684
- Species identification of the forensically important flies in Iwate prefecture, Japan based on mitochondrial cytochrome oxidase gene subunit I (COI) sequences.Legal Medicine. 2005; 7: 175-178
- Genetic relationships between blowflies (Calliphoridae) of forensic importance.Forensic Science International. 2001; 120: 116-123
- Partial sequencing of the cytochrome oxydase b subunit gene I: a tool for the identification of European species of blow flies for postmortem interval estimation.Journal of Forensic Sciences. 2000; 45: 820-823
- The utility of mitochondrial DNA sequences for the identification of forensically important blowflies (Diptera: Calliphoridae) in southeastern Australia.Forensic Science International. 2001; 120: 60-67
- DNA-based identification of forensically important Chrysomyinae (Diptera: Calliphoridae).Forensic Science International. 2001; 120: 110-115
- A DNA-based approach to the identification of insect species used for postmortem interval estimation.Journal of Forensic Sciences. 1994; 39: 418-427
- Application of DNA-based methods in forensic entomology.Annual Review of Entomology. 2008; 53: 103-120
- Validation of a DNA-based method for identifying Chrysomyinae (Diptera: Calliphoridae) used in a death investigation.International Journal of Legal Medicine. 2007; 121: 1-8
- The application of mitochondrial DNA cytochrome oxidase II gene for the identification of forensically important blowflies in western China.American Journal of Forensic Medicine and Pathology. 2007; 28: 308-313
- PCR-RFLP identification of Diptera (Calliphoridae, Muscidae and Sarcophagidae)—a generally applicable method.Journal of Forensic Sciences. 2003; 48: 783-785
- Species identification of some common necrophagous flies in Guangdong province, southern China based on the rDNA internal transcribed spacer 2 (ITS2).Forensic Science International. 2008; 175: 17-22
- Identification of forensically important Chrysomya (Diptera: Calliphoridae) species using the second ribosomal internal transcribed spacer (ITS2).Forensic Science International. 2008; 177: 238-247
- A global study of forensically significant calliphorids: implications for identification.Forensic Science International. 2008; 177: 66-76
- Species-level paraphyly and polyphyly: frequency, causes, and consequences, with insights from animal mitochondrial DNA.Annual Review of Ecology Evolution and Systematics. 2003; 34: 397-423
- Paraphyly in Hawaiian hybrid blowfly populations and the evolutionary history of anthropophilic species.Insect Molecular Biology. 2002; 11: 141-148
- The evolution of myiasis in blowflies (Calliphoridae).International Journal for Parasitology. 2003; 33: 1105-1113
- Phylogenetic analysis of forensically important Lucilia flies based on cytochrome oxidase I sequence: a cautionary tale for forensic species determination.International Journal of Legal Medicine. 2007; 121: 229-233
- DNA barcoding: promise and pitfalls.PLOS Biology. 2004; 2: 1529-1531
- The evolution of myiasis in humans and other animals in the Old and New Worlds (part II): biological and life-history studies.Trends in Parasitology. 2006; 22: 181-188
- Development of monoclonal antibody-based immunological assays for the detection of live propagules of Rhizoctonia solani in soil.Plant Pathology. 1993; 42: 763-773
- Rapid accurate field diagnosis of Indian visceral leishmaniasis.Lancet. 1998; 351: 563-565
- HIV diagnostic tests: an overview.Contraception. 2004; 70: 141-147
- Rapid diagnostic tests for malaria parasites.Clinical Microbiology Reviews. 2002; 15: 66
- A rapid point of care immunoswab assay for SARS-CoV detection.Journal of Virological Methods. 2008; 152: 77-84
- Development of an immunochromatographic lateral-flow device for rapid serodiagnosis of invasive aspergillosis.Clinical and Vaccine Immunology. 2008; 15: 1095-1105
Article info
Publication history
Published online: February 24, 2009
Footnotes
☆Paper Presented at the DNA in Forensics Meeting, Ancona, 27–30 May, 2008.
Identification
Copyright
© 2009 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
