Abstract
Methylation of CpG dinucleotides was investigated in five regions by bisulphite treatment
of gDNA, PCR and cloning/sequencing. The gDNA was prepared from peripheral blood,
saliva, semen, nails and hair from the head. In gDNA from peripheral blood, three
regions were investigated in 16, 23 and 24 individuals, respectively (Fig. 2). In gDNA from other sources, three or five regions were investigated in five individuals
(Fig. 3). In many of the sequenced fragments, all the CpG dinucleotides were either methylated
or not, which support the idea that the parental origin of an allele may be determined
by the methylation status of the allele. However, the methylation of CpG dinucleotides
varies across the fragment in some of the sequenced fragments, especially from semen
samples, which indicate that it may be difficult to determine the parental origin
from some gDNA sources by restriction-enzyme analysis (DMPA method).
Keywords
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References
- A rapid, PCR based test for differential molecular diagnosis of Prader-Willi and Angelman syndromes.J. Med. Genet. 1998; 35: 472-475
- A census of mammalian imprinting.TRENDS Genet. 2005; 21: 458-465
- Novel paternity testing by distinguishing parental alleles at a VNTR locus in the differentially methylated region upstream of the human H19 gene.J. Forensic Sci. 2003; 48: 1275-1279
- Parentally imprinted allele typing at a short tandem repeat locus in intron 1a of imprinted gene KCNQ1.Legal Med. 2006; 8: 139-143
- Study on the application of parent-of-origin specific DNA methylation markers to forensic genetics.Forensic Sci. Int. 2005; 154: 122-127
- Parentally imprinted allele (PIA) typing in the differentially methylated region upstream of the human H19 gene.Forensic Sci. Int. Genet. 2008; 2: 286-291
- A parent-of-origin detectable polymorphism in the hypermethylated region upstream of the human H19 gene.Int. J. Legal Med. 2004; 118: 158-162
- Investigation on methylated genetic markers allowing selectively discrimination of either parental allele.DNA Polymorphism. 2004; 12 (in Japanese): 217-220
- Investigation of discrimination method for DMPA (differentially methylated parental allele).DNA Polymorphism. 2005; 13 (in Japanese): 53-56
- Investigation of discrimination method for DMPA (differentially methylated parental allele) II.DNA Polymorphism. 2006; 14 (in Japanese): 41-45
- CpG islands in vertebrate genomes.J. Mol. Biol. 1987; 196: 261-282
- Comprehensive analysis of CpG islands in human chromosomes 21 and 22.Proc. Natl. Acad. Sci. U.S.A. 2002; 99: 3740-3745
- Dependence of McrBC cleavage on distance between recognition elements.Biol. Chem. 1998; 379: 611-616
- A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.Proc. Natl. Acad. Sci. U.S.A. 1992; 89: 1827-1831
- Symmetric ad asymmetric DNA methylation in the human IGF2-H19 imprinted region.Genomics. 2000; 64: 132-143
- DHPLC-based method for DNA methylation analysis of differential methylated regions from imprinted genes.BioTechniques. 2003; 34: 356-362
- Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients.Hum. Mol. Genet. 2007; 16: 2541-2551
Article info
Publication history
Published online: March 16, 2009
Accepted:
February 9,
2009
Received in revised form:
January 28,
2009
Received:
September 27,
2008
Identification
Copyright
© 2009 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
