Research Article| Volume 4, ISSUE 2, P95-103, February 2010

Authentication of forensic DNA samples


      Over the past twenty years, DNA analysis has revolutionized forensic science, and has become a dominant tool in law enforcement. Today, DNA evidence is key to the conviction or exoneration of suspects of various types of crime, from theft to rape and murder. However, the disturbing possibility that DNA evidence can be faked has been overlooked. It turns out that standard molecular biology techniques such as PCR, molecular cloning, and recently developed whole genome amplification (WGA), enable anyone with basic equipment and know-how to produce practically unlimited amounts of in vitro synthesized (artificial) DNA with any desired genetic profile. This artificial DNA can then be applied to surfaces of objects or incorporated into genuine human tissues and planted in crime scenes. Here we show that the current forensic procedure fails to distinguish between such samples of blood, saliva, and touched surfaces with artificial DNA, and corresponding samples with in vivo generated (natural) DNA. Furthermore, genotyping of both artificial and natural samples with Profiler Plus® yielded full profiles with no anomalies. In order to effectively deal with this problem, we developed an authentication assay, which distinguishes between natural and artificial DNA based on methylation analysis of a set of genomic loci: in natural DNA, some loci are methylated and others are unmethylated, while in artificial DNA all loci are unmethylated. The assay was tested on natural and artificial samples of blood, saliva, and touched surfaces, with complete success. Adopting an authentication assay for casework samples as part of the forensic procedure is necessary for maintaining the high credibility of DNA evidence in the judiciary system.


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        • Butler J.M.
        Forensic DNA Typing. Biology, Technology and Genetics of STR Markers.
        2nd ed. Elsevier Academic Press, Burlington2005
        • Jeffreys A.J.
        • Wilson V.
        • Thein S.L.
        Individual-specific ‘fingerprints’ of human DNA.
        Nature. 1985; 316: 76-79
        • Jobling M.A.
        • Gill P.
        Encoded evidence: DNA in forensic analysis.
        Nat. Rev. Genet. 2004; 5: 739-751
        • Morling N.
        Forensic genetics.
        Lancet. 2004; 364: s10-s11
        • Reilly P.
        Legal and public policy issues in DNA forensics.
        Nat. Rev. Genet. 2001; 2: 313-317
        • Opar A.
        Crime and punishment.
        Nat. Med. 2006; 12: 1110-1111
        • Lynch M.
        God's signature: DNA profiling, the new gold standard in forensic science.
        Endeavor. 2003; 27: 93-97
        • Saks M.J.
        • Koehler J.J.
        The coming paradigm shift in forensic identification science.
        Science. 2005; 309: 892-895
      1. Stirling D. Bartlett J.M.S. PCR Protocols (Methods in Molecular Biology). 2nd edition. Humana Press, New Jersey2003
        • Sambrook J.
        Molecular Cloning: A Laboratory Manual.
        Cold Spring Harbor Laboratory Press, New York2001
        • Lasken R.S.
        • Egholm M.
        Whole genome amplification: abundant supplies of DNA from precious samples or clinical specimens.
        Trends Biotechnol. 2003; 21: 531-535
        • Panelli S.
        • Damiani G.
        • Espen L.
        • Micheli G.
        • Sgaramella V.
        Towards the analysis of the genomes of single cells: further characterisation of the multiple displacement amplification.
        Gene. 2006; 372: 1-7
        • Miranda T.B.
        • Jones P.A.
        DNA methylation: the nuts and bolts of repression.
        J. Cell Physiol. 2007; 213: 384-390
        • Hashimshony T.
        • Zhang J.
        • Keshet I.
        • Bustin M.
        • Cedar H.
        The role of DNA methylation in setting up chromatin structure during development.
        Nat. Genet. 2003; 34: 187-192
        • Bird A.
        DNA methylation patterns and epigenetic memory.
        Genes Dev. 2002; 16: 6-21
        • Dean F.B.
        • Hosono S.
        • Fang L.
        • Wu X.
        • Faruqi A.F.
        • Bray-Ward P.
        • Sun Z.
        • Zong Q.
        • Du Y.
        • Du J.
        • Driscoll M.
        • Song W.
        • Kingsmore S.F.
        • Egholm M.
        • Lasken R.S.
        Comprehensive human genome amplification using multiple displacement amplification.
        Proc. Natl. Acad. Sci. U.S.A. 2002; 99: 5261-5266
        • Fritsch E.F.
        • Maniatis T.
        • Sambrook J.
        Molecular Cloning: A Laboratory Manual.
        2nd ed. Cold Spring Harbor Laboratory Press, New York1989
        • Butler J.M.
        • Schoske R.
        • Valone P.M.
        • Redman J.W.
        • Kline M.C.
        Allele frequencies for 15 autosomal STR loci on U.S. Caucasian, African American, and Hispanic populations.
        J. Forensic Sci. 2003; 48: 908-911
        • Frommer M.
        • McDonald L.E.
        • Millar D.S.
        • Collis C.M.
        • Watt F.
        • Grigg G.W.
        • Molloy P.L.
        • Paul C.L.
        A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.
        Proc. Natl. Acad. Sci. U.S.A. 1992; 89: 1827-1831
        • Bond J.W.
        • Hammond C.
        The value of DNA material recovered from crime scenes.
        J. Forensic Sci. 2008; 53: 797-801
        • Levitt M.
        Forensic databases: benefits and ethical and social costs.
        Br. Med. Bull. 2007; 83: 235-248
        • Sobrino B.
        • Brion M.
        • Carracedo A.
        SNPs in forensic genetics: a review on SNP typing methodologies.
        Forensic Sci. Int. 2005; 154: 181-194
        • Butler J.M.
        • Coble M.D.
        • Vallone P.M.
        STRs vs. SNPs: thoughts on the future of forensic DNA testing.
        Forensic Sci. Med. Pathol. 2007; 3: 200-205
        • Babol-Pokora K.
        • Berent J.
        SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues.
        Acta Biochim. Pol. 2008; 55: 815-819
        • Nakahara H.
        • Hosono N.
        • Kitayama T.
        • Sekiguchi K.
        • Kubo M.
        • Takahashi A.
        • Nakamura Y.
        • Yamano Y.
        • Kai K.
        Automated SNPs typing system based on the invader assay.
        Leg. Med. 2009; 11: S111-S114
        • Gill P.
        • Brenner C.H.
        • Buckleton J.S.
        • Carracedo A.
        • Krawczak M.
        • Mayr W.R.
        • Morling N.
        • Prinz M.
        • Schneider P.M.
        • Weir B.S.
        DNA commission of the International Society of Forensic Genetics: recommendations on the interpretation of mixtures.
        Forensic Sci. Int. 2006; 160: 90-101
        • Shinde D.
        • Lai Y.
        • Sun F.
        • Arnheim N.
        Taq DNA polymerase slippage mutation rates measured by PCR and quasi-likelihood analysis: (CA/GT)n and (A/T)n microsatellites.
        Nucleic Acids Res. 2003; 31: 974-980
        • Forster L.
        • Thomson J.
        • Kutranov S.
        Direct comparison of post-28-cycle PCR purification and modified capillary electrophoresis methods with the 34-cycle “low copy number” (LCN) method for analysis of trace forensic DNA samples.
        Forensic Sci. Int. Genet. 2008; 2: 318-328

      Linked Article

      • Authentication of forensic DNA samples [Forensic Sci. Int. Genet. (2009)]
        Forensic Science International: GeneticsVol. 5Issue 3
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          Frumkin et al. in their article entitled “Authentication of forensic DNA samples” Forensic Sci. Int. Genet. (2009) doi:10.1016/j.fsigen.2009.06.009 , describe the possibility of faking the results of forensic DNA analysis by applying artificial DNA on objects at crime scenes. In addition, they propose an authentification procedure for distinguishing between natural and artificial DNA.
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      • Authentication of forensic DNA samples
        Forensic Science International: GeneticsVol. 5Issue 3
        • Preview
          In their recent paper entitled “Authentication of Forensic DNA samples” Frumkin et al. [1] describe not only how they are suggesting to authenticate DNA found at a crime scene but also explain in detail how to fabricate samples and plant DNA of specific individuals as false evidence. We believe that this type of information should have been handled more carefully and have several concerns with this publication.
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