Advertisement
Short communication| Volume 6, ISSUE 1, P124-131, January 2012

Download started.

Ok

Developmental validation of the PowerPlex® ESX 16 and PowerPlex® ESX 17 Systems

      Abstract

      We describe the developmental validation study performed on the PowerPlex® ESX 16 (European Standard Extended 16) and the PowerPlex® ESX 17 Systems, part of a suite of four new DNA profiling kits developed by Promega in response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe. The PowerPlex® ESX 16 System combines the 11 loci compatible with the UK National DNA Database, contained within the AmpFlSTR® SGM Plus® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to incorporate these five new loci as mini- and midi-STRs while maintaining the loci found in the AmpFlSTR® SGM Plus® kit as standard size. The PowerPlex® ESX 17 System amplifies the same loci as the PowerPlex® ESX 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR® SGM Plus® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex® ESX 16 and ESX 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. In mixture analysis, a range of 52–95% of unique minor contributor alleles was observed at 19:1 mixture ratios where only 25 pg of the minor component was present. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of information obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Forensic Science International: Genetics
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

      1. http://register.consilium.europa.eu/pdf/en/05/st10/st10900.en05.pdf (accessed 01.02.10).

      2. Council Decisions 2008/615/JHA and 2008/616/JHA. http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2008:210:0001:0011:EN:PDF (accessed 01.02.10), and http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2008:210:0012:0072:EN:PDF (accessed 01.02.10).

      3. Off. J. Eur. Union Council Resolution, 30th November 2009 (2009/C 296/01), 5th December 2009. http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:C:2009:296:0001:0003:EN:PDF (accessed 27.04.10).

      4. V.C. Tucker, A.J. Hopwood, C.J. Sprecher, R.S. McLaren, D.R. Rabbach, M.G. Ensenberger, J.M. Thompson, D.R. Storts, Developmental validation of the PowerPlex® ESI 16 and PowerPlex® ESI 17 Systems: STR multiplexes for the new European standard, Forensic Sci. Int. Genet. (in press).

      5. Revised validation guidelines, Scientific Working Group on DNA Analysis Methods (SWGDAM).
        Forensic Sci. Commun. 2004; 6 (http://www2.fbi.gov/hq/lab/fsc/backissu/july2004/standards/2004_03_standards02.htm (accessed 22.02.11))
        • DNA Advisory Board
        Quality assurance standards for forensic DNA testing laboratories.
        Forensic Sci. Commun. 2008; 10 (http://www2.fbi.gov/hq/lab/fsc/backissu/oct2008/standards/2008_10_standards01b.htm (accessed 22.02.11))
      6. C.R. Hill, D.L. Duewer, M.C. Kline, C.J. Sprecher, R.S. McLaren, D.R. Rabbach, B.E. Krenke, D.R. Storts, J.M. Butler, Concordance and populations studies along with stutter and peak height ratio analysis for the PowerPlex® ESX 17 and ESX 17 Systems, Forensic Sci. Int. Genet. (in press).

        • Cotton E.A.
        • Allsop R.F.
        • Guest J.L.
        • Frazier R.R.E.
        • Koumi P.
        • Callow I.P.
        • Seager A.
        • Sparkes R.L.
        Validation of the AmpFlSTR® SGM Plus® system for use in forensic casework.
        Forensic Sci. Int. 2000; 112: 151-161
        • Parys-Proszek A.
        • Kupiec T.
        • WolaDska-Nowak P.
        • Branicki W.
        Genetic variation of 15 autosomal STR loci in a population sample from Poland.
        Legal Med. 2010; 12: 246-248
      7. A. Berti, F. Brisighelli, A. Bosetti, E. Pilli, C. Trapani, V. Tullio, C. Franchi, G. Lago, C. Capelli, Allele frequencies of the new European Standard Set (ESS) loci in the Italian population, Forensic Sci. Int. Genet. (in press).

      8. M. Poetsch, K. Bayer, Z. Ergin, M. Milbrath, T. Schwark, N. von Wurmb-Schwark, First experiences using the new PowerPlex® ESX17 and ESI17 kits in casework analysis and allele frequencies for two different regions in Germany, Int. J. Legal Med. (in press).

      9. A. Molnár, A. Zalán, G. Horváth, H. Pamjav, Allele distribution of the new European Standard Set (ESS) loci in the Hungarian population, Forensic Sci. Int. Genet. (in press).

      10. I. Yurrebaso, J.A. Ajuriagerra, A. Alday, I. Lezama, J.A. Pérez, E. Romón, I. Uriarte, O. García, Allele frequencies and concordance study between the Identifiler and the PowerPlex ESX17 systems in the Basque Country population, Forensic Sci. Int. Genet. (in press).

      11. G. Mertens, S. Rand, K. Moordtgat, E. Cardoen, I. De Bruyn, N. Mommers, G. Leijnen, E. Jehaes, W. Jacobs, Population genetic analysis of Moroccans residing in Belgium using 16 autosomal STRs of the PowerPlex ESI 17 multiplex, Forensic Sci. Int. Genet. (in press).

      12. M. Poetsch, Z. Ergin, K. Bayer, D. El-Mostaqim, N. Rakotomavo, E.N. L. Browne, C. Timmann, R.D. Horstmann, T. Schwark, N. von Wurmb-Schwark, The new Powerplex® ESX17 and ESI17 kits in paternity and maternity analyses involving people from Africa—including allele frequencies for three African populations, Int. J. Legal Med. (in press).

        • Dixon L.A.
        • Murray C.M.
        • Archer E.J.
        • Dobbins A.E.
        • Koumi P.
        • Gill P.
        Validation of a 21-locus autosomal SNP multiplex for forensic identification purposes.
        Forensic Sci. Int. 2005; 154: 62-77
        • Lowe A.
        • Murray C.
        • Whitaker J.
        • Tully G.
        • Gill P.
        The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces.
        Forensic Sci. Int. 2002; 129: 25-34
        • McNevin D.
        • Wilde-Wilson L.
        • Robertson J.
        • Kyd J.
        • Lennard C.
        Short tandem repeat (STR) genotyping of keratinised hair: Part 2. An optimised genomic DNA extraction procedure reveals donor dependence of STR profiles.
        Forensic Sci. Int. 2005; 153: 247-259
        • Whitaker J.P.
        • Cotton E.A.
        • Gill P.
        A comparison of the characteristics of profiles produced with the AmpFlSTR ® SGM Plus™ Multiplex System for both standard and low copy number (LCN) STR DNA analysis.
        Forensic Sci. Int. 2001; 123: 215-223
        • Gill P.
        • Whitaker J.
        • Flaxman C.
        • Brown N.
        • Buckleton J.
        An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA.
        Forensic Sci. Int. 2000; 112: 17-40
      13. R. Green, J. Mulero, R. Lagace, W. Norona, C.-W. Chang, N. Oldroyd, L. Hennessy Developmental Validation of the AmpFSTR® NGM™ Kit, a Robust and Highly Discriminatory STR Multiplex 2009 Poster, http://marketing.appliedbiosystems.com/images/Product_Microsites/NGM/downloads/Green_et_al_NGM_Kit_Dev_Val_ISHI_2009.pdf (accessed 11.11.10).

      14. C. Phillips, L. Fermandez-Formoso, M. Garcia-Magarinos, L. Porras, T. Tvedebrink, J. Amigo, M. Fondevila, A. Gomez-Tato, J. Alvarez-Dios, A. Freire-Aradas, A. Gomez-Carballa, A. Mosquera-Miguel, A. Carracedo, M.V. Lareu, Analysis of global variability in 15 established and 5 new European Standard Set (ESS) STRs using the CEPH human genome diversity panel, Forensic Sci. Int. Genet. (in press).

      15. K.L. O’Connor, C.R. Hill, P.M. Vallone, J.M. Butler, Linkage disequilibrium analysis of D12S391 and vWA in U.S. population and paternity samples, Forensic Sci. Int. Genet. (in press).