Abstract
In this study, we tested the first commercial kit with insertion/deletion (indel)
polymorphisms, the Mentype® DIPplex PCR Amplification Kit (DIPplex kit). A total of 30 biallelic autosomal indels
and Amelogenin were amplified with the DIPplex kit. All loci were amplified in one
PCR multiplex and all amplicon lengths were shorter than 160 bp. Full indel profiles were generated from as little as 100 pg of DNA. A total of 117 individuals from Danish paternity cases were successfully
typed. No deviation from Hardy–Weinberg equilibrium was observed for any of the indels.
The combined mean match probability was 3.3 × 10−13, the mean paternity exclusion probability was 99.7% and the typical paternity indices
for trios and duos were 2350 and 165, respectively. Furthermore, we typed five highly
degraded DNA samples with the DIPplex kit, the AmpFlSTR® SGM Plus kit and the AmpFlSTR® SEfiler Plus kit. Full indel profiles were obtained with the DIPplex kit, whereas
only partial profiles were obtained with the STR kits. In general, the DIPplex kit
performed well and it would be a valuable assay for forensic genetic testing, especially
in crime cases with partially degraded DNA or low amounts of template DNA. However,
some difficulties with pull-ups were observed at DNA concentrations of 1000 pg. Rearrangement of the allele windows by changing the lengths of some of the PCR
primers would greatly improve the assay, and more robustness towards higher amounts
of DNA would allow the use of the DIPplex kit without prior quantification of the
samples.
Keywords
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References
- Primer3 on the WWW for general users and for biologist programmers.Methods Mol. Biol. 2000; 132: 365-386
- Arlequin (version 3.0): an integrated software package for population genetics data analysis.Evol. Bioinform. Online. 2005; 1: 47-50
- Performing the exact test of Hardy–Weinberg proportion for multiple alleles.Biometrics. 1992; 48: 361-372
- A simple sequentially rejective multiple test procedure.Scand. J. Stat. 1979; 6: 65-70
- Rectangular confidence regions for means of multivariate normal distributions.J. Am. Stat. Assoc. 1967; 62: 626-633
C. Brenner, DNAview, http://dna-view.com/dnaview.htm.
- Low template STR typing: effect of replicate number and consensus method on genotyping reliability and DNA database search results.Forensic Sci. Int. Genet. 2010; https://doi.org/10.1016/j.fsigen.2010.06.006
- An investigation of the robustness of the consensus method of interpreting low-template DNA profiles.Forensic Sci. Int. Genet. 2010; https://doi.org/10.1016/j.fsigen.2010.08.010
- A report of the 2002–2008 paternity testing workshops of the English speaking working group of the International Society for Forensic Genetics.Forensic Sci. Int. Genet. 2009; 3: 214-221
- Standards for Relationship Testing Laboratories.9th edition. 2009
- Validation of the AMPFlSTR MiniFiler PCR amplification kit for use in forensic casework.J. Forensic Sci. 2009; 54: 1046-1054
- A multiplex assay with 52 single nucleotide polymorphisms for human identification.Electrophoresis. 2006; 27: 1713-1724
- Autosomal SNP typing of forensic samples with the GenPlex HID System: results of a collaborative study.Forensic Sci. Int. Genet. 2010; https://doi.org/10.1016/j.fsigen.2010.06.007
Article info
Publication history
Published online: September 07, 2011
Accepted:
August 5,
2011
Received in revised form:
July 25,
2011
Received:
May 30,
2011
Identification
Copyright
© 2011 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
