The AmpFℓSTR® NGM™ PCR Amplification Kit includes 15 STRs loci and 12 of them (D3S1358, VWA, D8S1179,
D21S11, D18S51, TH01, FGA, D1S1656, D2S441, D10S1248, D12S391 and D22S1045) are part
of the European Standard Set (ESS). Five of these loci, D10S1248, D22S1045, D2S441,
D1S1656, D12S391, were recommended by the ENFSI and EDNAP groups to be adopted for
the analysis of degraded DNA samples, in order to improve the power of national databases
and to encourage standardization within Europe [
1
,
2
,
3
,
4
,
5
]. Allele frequencies and other forensically relevant statistic data for the STR loci
included in the AmpF2STR® NGM™ PCR Amplification Kit, were generated. No significant deviations from Hardy–Weinberg
expectations were found.Keywords
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References
- The evolution of DNA databases—recommendations for new European STR loci.Forensic Sci. Int. 2006; 156: 242-244
- New multiplexes for Europe. Amendments and clarification of strategic development.Forensic Sci. Int. 2006; 163: 155-157
- A highly variable STR at the D12S391 locus.Int. J. Legal Med. 1996; 109: 134-138
- Sequence variation of a hypervariable short tandem repeat at the D1S1656 locus.Int. J. Legal Med. 1998; 111: 244-247
- Characterization of new miniSTR loci to aid analysis of degraded DNA.J. Forensic Sci. 2005; 50: 43-53
- CHELEX® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.Biotechniques. 1991; 10: 506-513
- Schneider, Arlequin ver. 3.0: an integrated software package for population genetics data analysis.Evol. Bioinform. Online. 2005; 1: 47-50
- Tools for analysis of population statistics.Profile DNA. 1999; ((free software distributed by the authors at website): 14-16
- Allele frequencies of six miniSTR loci (D10S1248, D14S1434, D22S1045, D4S2364, D2S441 and D1S1677) in a Spanish population.Forensic Sci. Int. 2007; 169: 252-254
- Italian population data for D1S1656, D3S1358, D8S1132, D10S2325, VWA, FES/FPS, and F13A01.Forensic Sci. Int. 2001; 123: 71-73
- Allele frequencies of the five miniSTR loci D1S1656, D2S441, D10S1248, D12S391 and D22S1045 in a German population sample.Forensic Sci. Int. Genet. 2010; 4: e159-e160
- Genetic variation of 15 autosomal STR loci in a population sample from Poland.Legal Med. 2010; : 246-248
- Analysis of global variability in 15 established and 5 new European Standard Set (ESS) STRs using the CEPH human genome diversity panel.Forensic Sci. Int. Genet. 2011; 5: 155-169
- Population genetic analyses of the NGM STR loci.Int. J. Legal Med. 2011; 125: 01-109
- Allele frequencies of the new European Standard Set (ESS) loci in the Italian population.Forensic Sci. Int. Genet. 2011; 5: 548-549
- Publication of population data for forensic purposes.Forensic Sci. Int. Genet. 2010; 4: 145-147
Article info
Publication history
Published online: October 17, 2011
Received:
May 16,
2011
Identification
Copyright
© 2011 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
