Abstract
DNA testing from mixed cell samples can be difficult to use successfully in criminal
investigations. Here, we present a method for the extraction of DNA from mixed bloodstains
involving plural contributors, after antibody-microbead captured cell separation.
This method, together with the multiplex short tandem repeat typing presented, has
proven highly successful in the recovery of DNA profiles corresponding to the ABO
blood type. Methodological steps include magnetic separation using leukocyte specific
CD45 antibody-coated microbeads and centrifugal separation of leukocyte agglutination
by ABO antibody. The detection results of variable mixed ratio showed that the target
DNA was detected accurately as low as 1:512 mixed ratio, regardless of the large amount
of the background DNA present. The method presented here is applicable to PCR-based
identification for various kinds of mixed samples.
Keywords
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Article info
Publication history
Published online: January 15, 2014
Accepted:
January 4,
2014
Received in revised form:
December 22,
2013
Received:
June 11,
2013
Identification
Copyright
© 2014 Published by Elsevier Inc.
