Techniques using molecular markers have been used to study the differentiation of plant species, including Cannabis sativa. However, it is a field not yet explored in Brazil, especially in forensic routine. Although the use of non-coding region between trnL and trnF genes was described in previous studies [
- Linacre A.
- Thorpe J.
Detection and identification of cannabis by DNA.
Forensic Sci. Int. 1998; 91: 71-76
- Tsai L.C.
- Hsieh H.M.
- Huang L.H.
- Linacre A.
- Lee J.C.I
Cannabis seed identification by chloroplast and nuclear DNA.
Forensic Sci. Int. 2006; 158: 250-251
3], herein we report a standardized protocol for PCR and DNA sequencing on the use of a different section of the chloroplast genes trnL and trnF, as a potential identifier DNA marker for C. sativa samples, seized by the Polícia Civil do Estado do Rio de Janeiro (PCERJ).
- Kohjyouma M.
- Lee I.J.
- Iida O.
- Kurihara K.
- Yamada K.
- Makino Y.
- Sekita A.
- Satake M.
Intraspecific variation in Cannabis sativa L. based on intergenic spacer region of chloroplast DNA.
Biol. Pharma. Bull. 2000; 23: 727-730
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- Detection and identification of cannabis by DNA.Forensic Sci. Int. 1998; 91: 71-76
- Cannabis seed identification by chloroplast and nuclear DNA.Forensic Sci. Int. 2006; 158: 250-251
- Intraspecific variation in Cannabis sativa L. based on intergenic spacer region of chloroplast DNA.Biol. Pharma. Bull. 2000; 23: 727-730
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- Molecular phylogenetics and character evolution of Cannabaceae.Taxon. 2013; 62: 473-485
- Molecular identification of forensically important blow fly species (Diptera: Calliphoridae) in Taiwan.J. Med. Entomol. 2004; 41: 47-57
Published online: October 14, 2014
Received: August 7, 2014
© 2014 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.