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Letter to the Editor| Volume 14, P201-202, January 2015

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Genetic identification of Cannabis sativa using chloroplast trnL-F gene

Published:October 14, 2014DOI:https://doi.org/10.1016/j.fsigen.2014.10.003
      Techniques using molecular markers have been used to study the differentiation of plant species, including Cannabis sativa. However, it is a field not yet explored in Brazil, especially in forensic routine. Although the use of non-coding region between trnL and trnF genes was described in previous studies [
      • Linacre A.
      • Thorpe J.
      Detection and identification of cannabis by DNA.
      ,
      • Tsai L.C.
      • Hsieh H.M.
      • Huang L.H.
      • Linacre A.
      • Lee J.C.I
      Cannabis seed identification by chloroplast and nuclear DNA.
      ,
      • Kohjyouma M.
      • Lee I.J.
      • Iida O.
      • Kurihara K.
      • Yamada K.
      • Makino Y.
      • Sekita A.
      • Satake M.
      Intraspecific variation in Cannabis sativa L. based on intergenic spacer region of chloroplast DNA.
      ], herein we report a standardized protocol for PCR and DNA sequencing on the use of a different section of the chloroplast genes trnL and trnF, as a potential identifier DNA marker for C. sativa samples, seized by the Polícia Civil do Estado do Rio de Janeiro (PCERJ).
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