- •Yfiler® testing in 47 spermatozoa negative digital and/or penile penetration cases.
- •Predominantly vulval and low vaginal casework samples tested.
- •30% of cases produced at least one sample with three or more Y-STR alleles.
- •21% of cases produced at least one sample with 10 or more Y-STR alleles.
- •Demonstrated the persistence of male DNA for at least 48 h.
This forensic casework trial involved Yfiler® testing samples from 47 digital and/or penile penetration cases where the medical examination had occurred within 48 h of the alleged incident and no spermatozoa had been detected following Sperm Elution©. 30% of these cases yielded at least one Y-STR profile comprising three or more alleles per profile and 21% yielded at least one Y-STR profile of ten or more alleles per profile. This trial further investigated the persistence of male DNA in different case types, the location of samples submitted for testing and whether samples from different locations benefit from being combined prior to testing. The data supports the use of Y-STR profiling to provide scientific evidence to investigate whether the alleged sexual activity had occurred as well as to obtain probative evidence in spermatozoa negative penetration cases.
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- Sperm elution: an improved two phase recovery method for sexual assault samples.Sci. Justice. 2013; 53: 28-33
Faculty of Forensic and Legal Medicine (FFLM), Recommendations for the collection of forensic specimens from complainants and suspects (July 2014), http://www.fflm.ac.uk.
- Use of a Y chromosome probe as an aid in the forensic proof of sexual assault.J. Forensic Sci. 2002; 47: 601-604
- Semen and seminal stains.Arch. Pathol. 1943; 35: 140-196
- Identification and isolation of male cells using fluorescence in situ hybridisation and laser microdissection, for use in the investigation of sexual assault.Forensic Sci. Int. Genet. 2007; 1: 247-252
- Multiplexing of Y-chromosome specific STRs and performance for mixed samples.Forensic Sci. Int. 1997; 85: 209-218
- Y-STR DNA amplification as biological evidence in sexually assaulted female victims with no cytological detection of spermatozoa.Forensic Sci. Int. 2002; 125: 212-216
- Analysis of non-suspect samples lacking visually identifiable sperm using a Y-STR 10-plex.J. Forensic Sci. 2005; 50: 1116-1118
- The efficiency of Y-chromosome markers in forensic trace analysis and their inclusion in the Austrian National DNA Database.Forensic Sci. Int. Genet. Suppl. Ser. 2013; 4: e172-e173
- STR analysis with epithelial cells in a rape case.Forensic Sci. Int. 2001; 118: 126-130
S. Trabuio, M. Franco, Impact of Yfiler® testing on the recovery of evidence in sexual assault investigations, in: Poster at the 2012 Australia and New Zealand Forensic Science Society Conference.
- Increased Efficiency of Forensic Y-STR Analysis with the New Powerplex Y23 System.Dept. of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charité-Universitätsmedizin, Berlin, Germany2012
Published online: October 23, 2014
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