Highlights
- •28-Cycle Identifiler analysis can be undertaken on X/Y-FISH epithelial cells.
- •75 or more LMD X/Y-FISH epithelial cells, is optimal for a 28 cycle Identifiler test.
- •For 30 or less LMD X/Y-FISH epithelial cells, 34 cycle SGM Plus is recommended.
- •Hb was not improved for Identifiler profiles from LMD X/Y-FISH epithelial cells.
Abstract
During the investigation of allegations of sexual assault, samples are frequently
encountered that contain DNA from a female and a male donor. These may represent contributions
of DNA from the complainant and potentially, the offender. Many semen stained samples
successfully undergo DNA analysis and interpretation using a differential extraction
method that separates sperm from the epithelial cells present in the stain. However,
for those mixed cell samples that contain only epithelial cells, separation of any
male cells from female cells is problematic. This paper describes the application
of fluorescent in situ hybridisation (FISH) for the gender identification of epithelial
cells and subsequent recovery of target cells using laser microdissection (LMD). The
profiling results obtained from samples of known cell numbers using the Identifiler™
multiplex at standard 28-cycle PCR conditions and, when cell numbers are low, the
SGM Plus™ multiplex at elevated 34-cycle PCR conditions (also known as Low Copy Number
DNA analysis (LCN)) are described.
Keywords
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Article info
Publication history
Published online: October 25, 2014
Accepted:
October 16,
2014
Received in revised form:
August 26,
2014
Received:
May 4,
2014
Identification
Copyright
© 2014 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
