The quality and quantity of DNA extracted from any forensic sample is of paramount importance for the downstream forensic analyses and ultimately in the resolution of a crime. The extraction procedure can therefore be considered as one of the most critical steps in the molecular manipulations to which evidentiary material is exposed as it is imperative that the integrity and identity of the sample is not jeopardised. The turnover time for laboratory procedures is also of importance for a case so as to meet legal time constraints and ultimately confine perpetrators of crime. To support this endeavour, a combination of forensic genetics armamentaria [i.e., high-throughput platforms for liquid handling and capillary electrophoresis, highly informative and sensitive multiplex human identification systems, laboratory information management systems (LIMS) etc.] cooperatively optimise the workflow and submission of case reports to the justice system within acceptable timeframes to expedite downstream police investigations [
- Budowle B.
- Van Daal A.
Extracting evidence from forensic DNA analyses: future molecular biology directions.
BioTechniques. 2009; 46: 339-350
2]. The present letter summarises the internal validation of the QIAamp DNA Investigator and QIAamp 96 DNA Swab BioRobot Kits for DNA extraction and the BioRobot Universal System an automated liquid handling platform developed by QIAGEN (Hilden, Germany) for forensic applications [
- Keating B.
- Bansal A.T.
- Walsh S.
- Millman J.
- Newman J.
- Budowle B.
- Eisenberg A.
- Donfack J.
- Gasparini P.
- Budimlija Z.
- Henders A.K.
- Chandrupatla H.
- Duffy D.L.
- Gordon S.D.
- Hysi P.
- Liu F.
- Medland S.E.
- Rubin L.
- Martin N.G.
- Spector T.D.
- Kayser M.
- International Visible Trait Genetics (VisiGen) Consortium
First all-in-one diagnostic tool for DNA intelligence: genome-wide inference of biogeographic ancestry, appearance, relatedness, and sex with Identitas v1 Forensic Chip.
Int. J. Legal Med. 2013; 127: 559-572
5]. The necessary experiments were performed in accordance with validation guidelines, quality assurance standards and manufacturer published methods to demonstrate that these commercially available DNA extraction methods perform as expected when they are implemented in our laboratory to extract DNA from both reference (high yield DNA) and crime scene (low yield DNA) samples [
General requirements for the competence of testing and calibration laboratories – EN ISO/IEC 17025.
7]. The validation parameters assessed in this study included accuracy, repeatability, sensitivity, contamination prevention, sample and data tracking, suitability of methods for sample diversity and a comparison of the manual and automated methodologies with respect to DNA extraction efficiency.
Scientific Working Group on DNA Analysis Methods – Validation Guidelines for DNA Analysis Methods.
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- Extracting evidence from forensic DNA analyses: future molecular biology directions.BioTechniques. 2009; 46: 339-350
- First all-in-one diagnostic tool for DNA intelligence: genome-wide inference of biogeographic ancestry, appearance, relatedness, and sex with Identitas v1 Forensic Chip.Int. J. Legal Med. 2013; 127: 559-572
- QIAamp DNA Investigator Handbook.April 2010
- QIAamp 96 DNA Swab BioRobot Kit Handbook.April 2010
- BioRobot Universal System User Manual Version 1.0.January 2007
- General requirements for the competence of testing and calibration laboratories – EN ISO/IEC 17025.2005
- Scientific Working Group on DNA Analysis Methods – Validation Guidelines for DNA Analysis Methods.2012
National Institute of Standards & Technology Certificate of Analysis Standard Reference Material 2391, PCR-Based DNA Profiling Standard, Certificate Issue Date: 11 August 201.
- Developmental validation of a multiplex qPCR assay for assessing the quantity and quality of nuclear DNA in forensic samples.Forensic Sci. Int. 2007; 170: 35-45
Technical Manual PowerPlex 16 System–Instructions for use of Products (DC6530 and DC6531).
- A comparison of methods for forensic DNA extraction: Chelex-100 and the QIAGEN DNA Investigator Kit (manual and automated).Forensic Sci. Int. Genet. 2012; 6: 282-285
- A comparison of DNA collection and retrieval from two swab types (cotton and nylon flocked swab) when processed using three QIAGEN extraction methods.J. Forensic Sci. 2012; 57: 713-717
- Comparison of two methods for isolating DNA from human skeletal remains for STR analysis.J. Forensic Sci. 2012; 57: 706-712
Published online: November 01, 2014
Accepted: October 24, 2014
Received in revised form: October 15, 2014
Received: August 22, 2014
© 2014 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.