Highlights
- •X-chromosome STRs analysis has a potential usefulness in paternity testing.
- •Haplotype frequencies in 200 unrelated Italian males have been estimated.
- •Comparison with European populations showed some minor differences.
- •Contribute to the statistical evaluation of the X-STRs polymorphism.
Abstract
Requests for solving complex kinship casework involving at least one female are increasing
and in these circumstances the analysis of X-chromosomal STR markers plays a relevant
role. Actually, it is well known the superior statistical power of X-STRs compared
to autosomal markers in solving relationship when two sisters or half-sisters are
involved and none of parents is available, in maternity testing or in cases involving
close relatives as alternative putative fathers. In addition, the possibility to amplify
more loci simultaneously and the strategy based on the analysis of four linkage groups
to obtain the X-haplotype provide a powerful and validated tool. Nevertheless, haplotypes
frequency distribution in different populations is still needed for calculation of
probabilities in relationship testing. Published haplotype frequencies from German
population data are available, but in different caseworks we found unreported X-haplotypes.
To enlarge the forensic X-chromosome database, we present haplotype frequencies and
other parameter of forensic interest obtained from 200 anonymous DNA samples of unrelated
Italian males for the four linkage groups included in the Investigator Argus X-12
kit. From the comparison of the Italian sample haplotype frequencies with other populations,
significant genetic distances were found with Asian and African populations, but not
with Europeans. Finally, casework examples of complex kinship analysis are presented.
Keywords
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Article info
Publication history
Published online: November 15, 2014
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© 2014 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
