Highlights
- •Bi-allelic insertion/deletion polymorphisms (InDels) were detected from challenged samples.
- •An analytical threshold was established based on an assessment of noise in the system.
- •It is possible to obtain complete InDel profiles from minute amounts of DNA.
- •InDel profiles may be used as a supplement where only a partial STR profile is obtained.
Abstract
This research focuses on detection of bi-allelic insertion/deletion polymorphisms
(InDels) from challenged samples using the Investigator DIPplex® Kit from Qiagen. The study included analyzing body fluids from humans, as well as
pristine and degraded samples. For the purpose of assessing species specificity, samples
from various animals were included. At first, an analytical threshold (AT) for the
detection of alleles was established based on an assessment of the noise in the system.
Then, InDel profiles were obtained from samples exposed to detrimental environmental
conditions, washed bloodstains, lipsticks, ChapStick®, ancient Croatian bone samples, and every day products such as toothbrushes and dental
floss. Concordant profiles were obtained from different body fluids of the same donor.
InDel profiles were also generated successfully when body fluids were deposited on
substrates and directly amplified without pre-treatment with buffer or washing reagents.
InDels can provide additional information when only partial STR profiles are generated
from challenged samples.
Keywords
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Article info
Publication history
Published online: December 04, 2014
Accepted:
November 25,
2014
Received in revised form:
November 23,
2014
Received:
October 19,
2014
Identification
Copyright
© 2014 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
